Fluorescent quantitative PCR detection kit of tumor multidrug resistance gene and detection method thereof

Abstract

The invention discloses a fluorescent quantitative PCR detection kit of tumor multidrug resistance gene and detection method thereof, wherein the kit comprises MDR1, MRP1, LRP and inner control GAPDH gene standard, sense / anti-sense primers for detecting the MDR1, MRP1, LRP and inner control GAPDH gene standard and fluorescent probes; the detection method of the kit comprises: extracting the cell total RNA of the sample to be detected, performing the reverse transcription of the RNA into cDNA, performing the fluorescent quantitative PCR of the MDR1, MRP1, LRP and inner control GAPDH gene standard, calculating the initial replication number of the MDR1, MRP1, LRP gene of the sample to be detected. The fluorescent quantitative PCR detection kit of tumor multidrug resistance gene and detection method can accurately, sensitively and specially detect the MDR1, MRP1, LRP gene at the same time and guide the tumor chemotherapeutic drugs, with features of quick and simple operation, easy standardization, low detection cost, suitable for the dynamic monitor of the multidrug resistance of the tumor patient.

Description

technical field [0001] The invention relates to a detection kit, in particular to a fluorescent quantitative PCR detection kit for tumor multi-drug resistance-related genes, and also relates to a detection method of the kit. Background technique [0002] Malignant tumor is a disease that seriously threatens human health, and its incidence has been increasing year by year in recent years. Chemotherapy is one of the effective means to cure malignant tumors and prevent their metastasis and recurrence. However, with the widespread use of anticancer drugs, the problem of tumor drug resistance has become increasingly prominent, which has become one of the main obstacles to effective treatment of tumors. According to the drug resistance characteristics of tumor cells, tumor drug resistance can be divided into two types: original drug resistance (PDR) and multidrug resistance (MDR). PDR only produces resistance to the induced original drug and does not produce cross-resistance to o...

AI Technical Summary

Problems solved by technology

[0007] At present, the detection of MDR mainly uses methods such as flow cytometry, Western Blot and immunohistochemistry to detect related proteins such as p-gp, MRP1 and LRP, but the above methods have problems such as cumbersome operation, difficult standardization and high detection cost. limited in application

Images