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Method for detecting time resolution of surface plasma resonance spectroscopy

A technology of surface plasmon and time resolution, applied in the field of time resolution detection of surface plasmon resonance spectroscopy, can solve the problems of long time, low time resolution, inability to detect rapid changes in SPR angle, etc., to achieve low cost, rapid detection, simple structure

Inactive Publication Date: 2011-03-30
CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this method takes a long time to complete an angle scan, and its time resolution is low, so it cannot detect rapid changes in the SPR angle.

Method used

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  • Method for detecting time resolution of surface plasma resonance spectroscopy
  • Method for detecting time resolution of surface plasma resonance spectroscopy
  • Method for detecting time resolution of surface plasma resonance spectroscopy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The time-resolution detection method of the SPR spectrum of embodiment 1 morphine molecule

[0036] The instrument used in the present invention is an electrochemical in-situ time-resolved surface plasmon resonance measuring instrument, see Niu Li, Yang Guifu, Li Fenghua, Yuan Fuyu, Feng Yunxiang, Wang Wei; Electrochemical in-situ time-resolved surface plasmon resonance measuring instrument; China Application number 2007100552596; on the basis of it, the inventor improved the light intensity detector of the instrument, and replaced the original photodiode with a dual-channel detector, which improved the time resolution and provided a time-resolved surface plasmon resonance spectrum rate detection method.

[0037] Such as figure 1 As shown, the device used in the time-resolution detection method of a surface plasmon resonance (SPR) spectrum of the present invention comprises a laser 1, a semi-cylindrical prism 2, a gold thin film 3, a dual-channel light intensity detect...

Embodiment 2

[0049] The time resolution detection method of the SPR spectrum of embodiment 2 methamphetamine

[0050] Apparatus used and steps 1-2 are all the same as in Example 1; then carry out the following steps:

[0051] 3. Pass through 1 mg / mL methamphetamine antibody solution to bond with the mercaptopropionic acid on the gold film 3, and bind the methamphetamine antibody to the surface of the gold film 3. Then pass through 0.05 mol / L phosphate buffer solution (PBS) with pH=7.0 to wash.

[0052] 4. Pass through 0.2mmol / L glycine solution to deactivate, reduce the non-specific binding of methamphetamine antibody and mercaptopropionic acid. Then pass through 0.05 mol / L phosphate buffer solution (PBS) with pH=7.0 to wash.

[0053] 5 Passing a methamphetamine solution greater than 0.5 μg / mL, and performing a time-resolution detection of a surface plasmon resonance spectrum for specific recognition between the methamphetamine molecule and the methamphetamine antibody.

[0054] 6. The ...

Embodiment 3

[0057] The time resolution detection method of the SPR spectrum of embodiment 3 amphetamine

[0058] Apparatus used and steps 1-2 are all the same as in Example 1; then carry out the following steps:

[0059] 3. Pass through 1 mg / mL amphetamine antibody solution to bond with the mercaptopropionic acid on the gold film 3, and bind the methamphetamine antibody to the surface of the gold film 3. Then pass through 0.05mol / L phosphate buffer solution (PBS) of pH=7.0 to rinse;

[0060] 4. Pass through 0.2mmol / L glycine solution to deactivate, reduce the non-specific binding of amphetamine antibody and mercaptopropionic acid. Then pass through 0.05mol / L phosphate buffer solution (PBS) of pH=7.0 to rinse;

[0061] 5. Injecting an amphetamine solution greater than 0.5 μg / mL to perform a time-resolution detection of a surface plasmon resonance spectrum for specific recognition between the amphetamine molecule and the amphetamine antibody.

[0062] 6. The value of (A1-A2) / (A1+A2) is d...

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Abstract

The invention relates to a method for detecting time resolution of surface plasmon resonance spectroscopy, the using instrument is an electrochemical in situ time resolution surface plasmon resonance measuring instrument, and two light cells with the interval of only 0.1-0.5mm are used as a dual-channel light intensity detector of a SPR instrument for detecting the change state of the SPR angle with time. The structure is simple, the response time can achieve 30 nanoseconds, the stability can achieve 0.5 per mill and the cost is very low. As the combination of small molecules with a fixed antibody on a gold chip is a very fast process, the ordinary means can not realize the dynamic detection of the whole combination process. The method can realize the rapid detection of the change value ofthe surface plasmon resonance angle, so as to lead the obtainment of the dynamic information of the combination of the small molecules with the antibody thereof to be possible. The detected object range of the method is the dynamic process of mutual identification of the small molecules with the antibody thereof.

Description

technical field [0001] The invention belongs to a time resolution detection method of surface plasmon resonance spectrum. Background technique [0002] Surface plasmon resonance (SPR) is a technique developed rapidly in recent years for the analysis of biomolecular interactions. It uses the principle that the incident light can resonate with the plasmon on the metal surface during total reflection to detect whether there is interaction between biomolecules and the kinetic parameters of the reaction. Surface plasmon resonance detection technology has the characteristics of high sensitivity, less samples required, no labeling of samples, and fast detection speed. It has been widely used in various fields such as immunology, proteomics, drug screening, protein-nucleic acid interaction, etc. Many kinetic data cannot be obtained by other methods. [0003] The current angle modulation SPR detection equipment uses the movement of the mechanical structure to change the incident an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/75G01N33/53
Inventor 袁福宇牛利王伟李风华李斐
Owner CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
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