Method for detecting time resolution of surface plasma resonance spectroscopy
A technology of surface plasmon and time resolution, applied in the field of time resolution detection of surface plasmon resonance spectroscopy, can solve the problems of long time, low time resolution, inability to detect rapid changes in SPR angle, etc., to achieve low cost, rapid detection, simple structure
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Embodiment 1
[0035] The time-resolution detection method of the SPR spectrum of embodiment 1 morphine molecule
[0036] The instrument used in the present invention is an electrochemical in-situ time-resolved surface plasmon resonance measuring instrument, see Niu Li, Yang Guifu, Li Fenghua, Yuan Fuyu, Feng Yunxiang, Wang Wei; Electrochemical in-situ time-resolved surface plasmon resonance measuring instrument; China Application number 2007100552596; on the basis of it, the inventor improved the light intensity detector of the instrument, and replaced the original photodiode with a dual-channel detector, which improved the time resolution and provided a time-resolved surface plasmon resonance spectrum rate detection method.
[0037] Such as figure 1 As shown, the device used in the time-resolution detection method of a surface plasmon resonance (SPR) spectrum of the present invention comprises a laser 1, a semi-cylindrical prism 2, a gold thin film 3, a dual-channel light intensity detect...
Embodiment 2
[0049] The time resolution detection method of the SPR spectrum of embodiment 2 methamphetamine
[0050] Apparatus used and steps 1-2 are all the same as in Example 1; then carry out the following steps:
[0051] 3. Pass through 1 mg / mL methamphetamine antibody solution to bond with the mercaptopropionic acid on the gold film 3, and bind the methamphetamine antibody to the surface of the gold film 3. Then pass through 0.05 mol / L phosphate buffer solution (PBS) with pH=7.0 to wash.
[0052] 4. Pass through 0.2mmol / L glycine solution to deactivate, reduce the non-specific binding of methamphetamine antibody and mercaptopropionic acid. Then pass through 0.05 mol / L phosphate buffer solution (PBS) with pH=7.0 to wash.
[0053] 5 Passing a methamphetamine solution greater than 0.5 μg / mL, and performing a time-resolution detection of a surface plasmon resonance spectrum for specific recognition between the methamphetamine molecule and the methamphetamine antibody.
[0054] 6. The ...
Embodiment 3
[0057] The time resolution detection method of the SPR spectrum of embodiment 3 amphetamine
[0058] Apparatus used and steps 1-2 are all the same as in Example 1; then carry out the following steps:
[0059] 3. Pass through 1 mg / mL amphetamine antibody solution to bond with the mercaptopropionic acid on the gold film 3, and bind the methamphetamine antibody to the surface of the gold film 3. Then pass through 0.05mol / L phosphate buffer solution (PBS) of pH=7.0 to rinse;
[0060] 4. Pass through 0.2mmol / L glycine solution to deactivate, reduce the non-specific binding of amphetamine antibody and mercaptopropionic acid. Then pass through 0.05mol / L phosphate buffer solution (PBS) of pH=7.0 to rinse;
[0061] 5. Injecting an amphetamine solution greater than 0.5 μg / mL to perform a time-resolution detection of a surface plasmon resonance spectrum for specific recognition between the amphetamine molecule and the amphetamine antibody.
[0062] 6. The value of (A1-A2) / (A1+A2) is d...
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