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Strain TX-4 used for preventing aphelenchoides besseyi or vegetable root knot nematode and preparation thereof

A technology for rice stem tip nematode and nematode disease, which is applied in the directions of nematicides, bacteria, biocides, etc., can solve the problems of biocontrol microorganisms and their preparations that have not been reported on vegetable root knot nematodes and rice stem tip nematodes, and achieves To ensure the effect of pollution-free prevention and control, large profit margins, and good prevention and control effects

Inactive Publication Date: 2009-10-07
ZHENJIANG AGRI SCI INST JIANGSU HILLY AREAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, no biocontrol microorganisms and their preparations have been reported for the simultaneous control of vegetable root-knot nematode and rice stem apical nematode

Method used

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  • Strain TX-4 used for preventing aphelenchoides besseyi or vegetable root knot nematode and preparation thereof
  • Strain TX-4 used for preventing aphelenchoides besseyi or vegetable root knot nematode and preparation thereof
  • Strain TX-4 used for preventing aphelenchoides besseyi or vegetable root knot nematode and preparation thereof

Examples

Experimental program
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Embodiment

[0027] The bacterial strain TX-4 for preventing and controlling rice stem nematode disease or vegetable nematode disease in this example can be extracted and preserved in China Center for Type Culture Collection on March 19, 2009, with the preservation number: CCTCC NO: M209038. The Latin scientific name is Paenibacillus spp. The strain has the following characteristics: the cells are rod-shaped, mesophytic, with spores, slightly enlarged cysts, oval, G+, and flagella. The colonies are light yellow, round, with neat edges and raised colonies. The fermentation broth can produce a variety of antibacterial active substances, and can secrete extracellular protease and cellulase.

[0028] The biocontrol bacterium agent prepared by the bacterial strain TX-4 of the prevention and treatment of rice stem nematode disease or vegetable nematode disease of the present embodiment, its preparation method comprises the following steps:

[0029] (A) Cultivation of primary seed culture medium...

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PUM

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Abstract

The invention discloses a strain TX-4 used for preventing diseases of aphelenchoides besseyi or vegetable root knot nematode and a preparation thereof, belonging to the technical field of preventing and treating diseases of crops and being specially used for biological prevention of the crops. The prevention result of field demonstration shows that the strain has the preventing effect of more than 90 percent to the aphelenchoides besseyi disease and has preventing effect of more than 70 percent to the vegetable root knot nematode disease. The strain TX-4 for preventing diseases of aphelenchoides besseyi or vegetable root knot nematode is bacillus with the Latin systematic name of Paenibacillus spp., the strain has been preserved in China Center for Type Culture Collection on March 19, 2009 and the preserving number is CCTCC No: M209038.

Description

technical field [0001] The invention relates to a bacterial strain and its preparation, more specifically to a bacterial strain TX-4 and its preparation for preventing and treating rice stem-point nematode or vegetable root-knot nematode disease, belonging to the technical field of crop disease prevention and treatment, and a biological agent for special crops. Prevention and treatment. Background technique [0002] Rice stem-point nematode (Aphelechoides besseyi Christie, 1942) belongs to the nematode phylum-Laterocauda--Aphelenchoides--Aphelenchoides--Aphelenchoides--Aphelenchoides Fuscher (Aphelenchoides Fuscher, 1894)-- Bessie worm (A. besseyi) causes dry apical nematode disease of rice and twist nematode disease of millet. In recent years, studies have proved that it is also the pathogenic bacteria that causes rice "spike head". About 2 billion kilograms of rice are lost every year due to rice stem nematode disease in my country. For the prevention and treatment of r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P5/00C12R1/01
Inventor 杨敬辉朱桂梅潘以楼陈宏州
Owner ZHENJIANG AGRI SCI INST JIANGSU HILLY AREAS
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