Processing method for artemia sporocyst

A technology of cysts and magnetic particles, applied in the field of Artemia nauplii, can solve the problems of heavy labor and the influence of the viability of cysts

Active Publication Date: 2013-01-23
INVE TECH NV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Another disadvantage is that the decapsulation process is very labor intensive and needs to be done with precision, otherwise cyst viability may be compromised

Method used

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  • Processing method for artemia sporocyst
  • Processing method for artemia sporocyst
  • Processing method for artemia sporocyst

Examples

Experimental program
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Effect test

Embodiment

[0041] All reagents and chemicals used in these experiments were of analytical grade and were from Sigma Aldrich (Belgium). Magnetite suspension (MS) was obtained by mixing ferrous chloride tetrahydrate (FeCl 2 .4H 2 O), ferric chloride hexahydrate (FeCl 3 .6H 2 O), sodium hydroxide (NaOH) and water with stirring. The mixture forms a suspension of Fe 3 o 4 nanoparticles. The preparation thus prepared contained about 0.1 g of Fe 3 o 4 / ml.

[0042] Magnetite powder (iron(II,III) oxide, Sigma Aldrich, powder <5 μm) (MP) was suspended in water before coating onto cysts.

[0043] To coat magnetic particles with the method of the invention, 5 ml aliquots of different coating materials (MS and MP) at different concentrations were mixed with 50 g of different cyst types (water content of cysts ± 10%). Positive coating results were evident from visual inspection of the cysts.

[0044] One liter cones were used in which 2 g of Artemia cysts were incubated in artificial seawa...

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Abstract

Cysts, in particular Artemia cysts, are incubated the in a hatching medium so that a portion of the cysts hatch and release free swimming live food organisms. The free swimming live food organisms have subsequently to be separated from the cysts which have not hatched. Before incubating the cysts, magnetic particles are applied onto the cysts so that these cysts can be attracted magnetically, in particular in the liquid hatching medium. This enables to separate the unhatched cysts and the empty cyst shells, coated with the magnetic particles, effectively from the free swimming live food organisms which do not have magnetic particles applied onto their outer surface. The invention also relates to the coated cysts.

Description

technical field [0001] The present invention relates to a method of treating cysts (cysts), in particular Artemia cysts (Artemia cysts), incubating said cysts in a liquid hatching medium to produce live food organisms, in particular Artemia nauplii ( Artemia nauplii). Background technique [0002] The production of Artemia nauplii as live food for fish and crustacean larvae is considered an essential operation for marine hatcheries. The production steps of Artemia nauplii usually include hatching, separation, enrichment, washing and harvesting, where harvesting and washing of Artemia nauplii usually requires sieves or concentrators / rinsers. The concentrator is in fact a filter that is used to separate the wastewater from the nauplii, removing excess water from the nauplii after hatching to a final volume suitable for distribution or storage, and washing away after enrichment Oily coating on nauplii. [0003] After hatching, Artemia nauplii need to be separated from unhatc...

Claims

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Application Information

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IPC IPC(8): A01K67/033
CPCA01K61/008A01K61/17Y02A40/81A23K50/00C12N3/00
Inventor G·罗姆鲍特L·A·J·万纽温豪弗E·M·J·纳森斯-福卡夸尔特
Owner INVE TECH NV
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