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Polypeptide for preparing anti-PINK1 polyclonal antiserum and application thereof

A polyclonal antiserum, antiserum technology, applied in applications, antibodies, recombinant DNA technology, etc., can solve problems such as unclear

Inactive Publication Date: 2009-09-09
CAPITAL UNIVERSITY OF MEDICAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

How PINK1 regulates these proteins to maintain cell survival is still not well understood

Method used

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  • Polypeptide for preparing anti-PINK1 polyclonal antiserum and application thereof
  • Polypeptide for preparing anti-PINK1 polyclonal antiserum and application thereof
  • Polypeptide for preparing anti-PINK1 polyclonal antiserum and application thereof

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Embodiment Construction

[0046] In the following examples, unless otherwise specified, the methods used are conventional methods, and the reagents used can be obtained from commercial sources.

[0047] 1. Expression and purification of recombinant human PINK1 for animal immunization

[0048] The kinase domain of human PINK1 consists of 425 amino acids, and its amino acid sequence is shown in sequence 1 in the sequence listing. Total RNA was extracted from normal human embryonic brain, reverse-transcribed into cDNA, and PCR-amplified with primer 1 (5`CCC AAG TTT GTT GTG ACC G3`) and primer 2 (3`TGT AAT TTC CCA CTC CCGTA5`) encoding PINK1 The DNA encoding the kinase domain of human PINK1 obtained by PCR amplification was inserted into the pET-28a vector to form the recombinant plasmid pET-28a-PINK1. Transform pET-28a-PINK1 into BL21(DE3) competent cells, take a single colony and inoculate into 10mL 2×YTA-amp liquid medium (each liter contains 16g peptone, 10g yeast extract, 5g NaCl and 0.1g ampicillin)...

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Abstract

The present invention discloses a polypeptide for preparing anti-PINK1 polyclonal antiserum and application thereof. The polypeptide of the invention is one polypeptide selected from a) and b): a) the polypeptide which is composed of amino acid sequence shown by the sequence 2 in a sequence table; and b) the polypeptide which is derived from the amino acid sequence of sequence 2 in the sequence table through substitution and / or absence and / or adding one or a plurality of amino acids. The invention also discloses the DNA molecule encoding the polypeptide. The anti-PINK1 polyclonal antiserum prepared according to the invention not only can be used for detecting Western blot, but also can be applied to immunohistochemical analysis. The anti-PINK1 polyclonal antiserum prepared according to the invention is of great significance in diagnosing and treating the disease related with PD and PINK1.

Description

technical field [0001] The invention relates to polypeptides for preparing anti-PINK1 polyclonal antiserum and applications thereof. Background technique [0002] Parkinson's disease (Parkinson's Desease PD) is the second most common neurodegenerative disease after senile dementia. Its clinical features are resting tremor, bradykinesia, and increased muscle tone. The typical neuropathological feature is the selective degeneration and loss of dopamine neurons in the substantia nigra compact area of ​​the midbrain and a significant decrease in striatal dopamine content. In addition, fibrous eosinophilic inclusion bodies——Lewy bodies) appeared in the cytoplasm of surviving neurons, and the components of Lewy bodies were ubiquitin, α-Synuclein, and PETEN-induced Altered cleavage and localization of PINK1 to aggresomes in the presence of proteasomal stress Journal of Neurochemistry 2006, 98, 156-169) . PD-related genes discovered so far include α-Synuclein, LRRK2, UCH-L1, par...

Claims

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Application Information

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IPC IPC(8): C12N9/12C12N15/54C12N15/63C12N5/10C12N1/00A61K39/395C12N15/11
Inventor 杨慧李尧华于顺李继胜
Owner CAPITAL UNIVERSITY OF MEDICAL SCIENCES
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