Apparatus for simultaneous lossless detection of cell and extracellular matrix component
A non-destructive detection and cell technology, applied in the direction of material excitation analysis, fluorescence/phosphorescence, etc., can solve the problems of inaccurate staining time, loss of accuracy of inspection results, inappropriate specimen handling, etc.
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[0007] Embodiments of the present invention will be further described below in conjunction with the accompanying drawings, so as to make the present invention more comprehensible.
[0008] Such as figure 1 As shown, the light emitted by the laser is incident on the scanning mirror group of the scanner through a two-color beam splitter, and the beam passing through the scanning mirror group is focused on the detection sample by the objective lens; the two-photon excitation fluorescence and the second harmonic generated by the laser and the detection sample The wave signal is collected by the same objective lens in reverse, and then incident on a half-transparent mirror by the aforementioned scanning mirror group and dichroic beam splitter, and the emitted signal light composed of two-photon excited fluorescence and the second harmonic signal is divided into two paths, One path converts the second harmonic signal of collagen fibers into electrical signal A through filter 1 and p...
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