Ammonia ion diagnosis/measuring reagent kit and ammonia ion concentration determination method

A kit and ion technology, applied in the field of ammonia diagnosis/determination kit and ammonia concentration determination, can solve the problem of undiscovered blood ammonia determination method and the like

Inactive Publication Date: 2009-06-24
SUZHOU ANJ BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Searching Chinese patents, only found 87105593.7 patent application which discloses a quick-frozen cup for measuring blood ammonia, but did not find an ideal method for measuring blood ammonia

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] The ammonia (ion) diagnostic / measurement reagent in this embodiment is a single reagent, including:

[0046] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0047] Stabilizer 500mmol / L

[0048] Reduced coenzyme 0.25mmol / L

[0049] Glutamine Synthetase 6000U / L

[0050] Pyruvate carboxylase 8000U / L

[0051] Malate dehydrogenase 10000U / L

[0052] Glutamic acid 10mmol / L

[0053] Adenosine triphosphate 3mmol / L

[0054] Oxaloacetic acid 5mmol / L

[0055] Pyruvate 3mmol / L

[0056] After all the reagents are dissolved and prepared, they are divided into bottles and freeze-dried to prepare dry powder reagents; before use, add pure water and use after reconstitution.

[0057] Set on the automatic biochemical analyzer: temperature 37℃, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test sub-wavelength 405nm, the volume ratio of the tested ammonia (ion) sample to the reagent is 1 / 25, the reaction direction is negative reaction (de...

Embodiment 2

[0060] The ammonia (ion) diagnostic / measurement reagent in this embodiment is a dual reagent, including:

[0061] Reagent 1

[0062] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0063] Stabilizer 50mmol / L

[0064] Reduced coenzyme 0.25mmol / L

[0065] Glutamic acid 10mmol / L

[0066] Adenosine triphosphate 3mmol / L

[0067] Oxaloacetic acid 5mmol / L

[0068] Pyruvate 3mmol / L

[0069] Reagent 2

[0070] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0071] Stabilizer 500mmol / L

[0072] Glutamine Synthetase 6000U / L

[0073] Pyruvate carboxylase 8000U / L

[0074] Malate dehydrogenase 10000U / L

[0075] After all the reagents are dissolved and prepared, they are divided into bottles to form a liquid double reagent, which can be used directly.

[0076] Set on the automatic biochemical analyzer: temperature 37℃, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test sub-wavelength 405nm, measured ammonia (ion) sampl...

Embodiment 3

[0079] The ammonia (ion) diagnosis / measurement reagent in this embodiment is three reagents, including:

[0080] Reagent 1

[0081] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0082] Stabilizer 50mmol / L

[0083] Reduced coenzyme 0.25mmol / L

[0084] Glutamic acid 10mmol / L

[0085] Adenosine triphosphate 3mmol / L

[0086] Oxaloacetic acid 5mmol / L

[0087] Pyruvate 3mmol / L

[0088] Reagent 2

[0089] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0090] Stabilizer 500mmol / L

[0091] Pyruvate carboxylase 8000U / L

[0092] Malate dehydrogenase 10000U / L

[0093] Reagent 3

[0094] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0095] Stabilizer 500mmol / L

[0096] Glutamine Synthetase 6000U / L

[0097] After all the reagents are dissolved and prepared, they are divided into bottles to make the third liquid reagent, which can be used directly.

[0098] When determining the concentration of ammonia (ion), set on the automati...

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PUM

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Abstract

The invention relates to a kit for diagnosing/measuring ammonia (ions) by utilizing the technologies of the enzymic colorimetry and the enzyme linked immunosorbent assay. The invention further relates to a method, a principle and the composition and the components of a reagent for measuring the concentration of ammonia (ions), and belongs to the technical field of medical/food/environmental inspection and measurement. The main components of the kit include a buffer solution, reduced coenzyme, glutamic acid, adenyl pyrophosphate, oxaloacetic acid, pyruvic acid, glutamine synthetase, pyruvate carboxylase, malic dehydrogenase and a stabilizer. Through mixing a sample and the reagent by a certain volume ratio, a series of enzymatic reactions occur, then the reactant is placed under an ultraviolet/visible light analyzer, and the velocity of the decrease in absorbance at 340 nm of the dominant wavelength is detected, thereby measuring the concentration of ammonia (ions).

Description

Technical field [0001] The present invention relates to an ammonia (ion) diagnostic / determination kit. At the same time, the present invention also relates to a method for determining the concentration of ammonia (ion), which belongs to the technical field of medical / food / environmental inspection and determination. Background technique [0002] Ammonia determination methods include trace diffusion method, ion exchange method, enzymatic method and ammonia electrode method. At present, the most widely used methods are enzymatic method and blood ammonia analyzer analysis method based on ion selective electrode. [0003] The diffusion method is to release NH after alkalization of the specimen 3 , Use acid to titrate the released ammonia, or use Nessler reaction to form a brown-yellow amalgam iodide for color comparison. These methods require alkalization, and the formation of endogenous ammonia affects their accuracy and precision. They are rarely used at present; ion exchange method...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N33/84C12Q1/00
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD
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