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Inocula for preventing apple fungous diseases and increasing yield, and special strain therefore and use thereof

A fungal disease and inoculum technology, applied in the inoculum for preventing and controlling fungal diseases of apples and promoting yield increase and its special strains and application fields, can solve the problems of environmental protection danger, apple export and development impact, etc., and achieve apple quality improvement, mu Increased yield and improved stress resistance

Active Publication Date: 2009-06-24
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The annual economic loss caused by ring rot fruit disease in my country is 3 billion to 5 billion yuan, and the number of spraying in production is also increasing year by year, which not only causes huge economic losses to farmers, but also has great impact on the export and development of apples. Great impact, but also bring potential danger to environmental protection

Method used

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  • Inocula for preventing apple fungous diseases and increasing yield, and special strain therefore and use thereof
  • Inocula for preventing apple fungous diseases and increasing yield, and special strain therefore and use thereof
  • Inocula for preventing apple fungous diseases and increasing yield, and special strain therefore and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1, the screening of active ingredient in bacterial agent

[0020] The composition of NA medium: beef extract 3g / L, peptone 5g / L, NaCl 5g / L, agar 20g / L. pH7.0~7.2. Sterilize under 0.11MPa pressure for 30min.

[0021] In areas seriously affected by apple ring spot, select healthy apple fruits and bark from fruit trees where apple ring spot occurs, rinse them with sterile water, weigh 5g each, and soak them in 75% ethanol by volume for 3 minutes , then soak the fruit with 1% by volume sodium hypochlorite solution for 3 minutes, soak the bark with 0.1% by mass mercuric chloride for 3 minutes, rinse with sterile water for 3 to 5 times, and rinse with sterile water for the last time Spread on PDA and NA medium plates, incubate at a constant temperature of 28°C for 3-4 days, and repeat 3 times for each treatment. If no colony grows on the medium plate, it indicates that the surface of the material is completely sterilized. Take the bark and leaves that have been ...

Embodiment 2

[0026] Embodiment 2, preparation and control effect thereof of the inoculant of preventing and treating apple fungal diseases

[0027] One, the preparation of the inoculant of preventing and treating apple fungal diseases

[0028] 1. Activation and expansion of strains

[0029] The composition of NA medium: beef extract 3g / L, peptone 5g / L, NaCl 5g / L, agar 20g / L. pH7.0~7.2. Sterilize for 30min at 121~125℃ and 0.11~0.14MPa pressure.

[0030] The Bacillus subtilis (Bacillus subtilis) KTB004 CGMCC №2816 and Bacillus pumilus (Bacillus pumilus) KTB005 CGMCC № 2817 screened in the above Example 1 were streak cultured with NA medium respectively, and cultured in an incubator at 30-32°C for 24h , Place the colonies that have been inspected without miscellaneous bacteria and grow neatly in an incubator at 30-32°C for 24-28 hours, observe with the naked eye, smear and stain the single colony with a full bacterial lawn to check that there are no miscellaneous bacteria, and the spores ar...

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PUM

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Abstract

The invention discloses a microbial inoculum for controlling apple fungus diseases. The active ingredients of the microbial inoculum comprise bacillus subtilis and bacillus pumilus. The microbial inoculum for controlling apple fungus diseases mainly can control ring rot of apple with the control efficiency being over 80%, and also can treat other fungus diseases; the control efficiency for apple powdery mildew is within the range of 30-60%, the control efficiency for red rot can reach 45%, the control efficiency for tan rot can reach 40%, and the control efficiency for alternaria leaf spot in apple can reach 42.33%; therefore, the average yield per mu of the apples is increased by 9.3%, the quality of the apples is obviously improved, and the resistance of the apples is remarkably enhanced.

Description

technical field [0001] The invention relates to a fungal agent for preventing and controlling fungal diseases of apples and promoting production, as well as special strains and applications thereof. Background technique [0002] Apple ring rot is a major disease in apple production, and has become a difficult problem for fruit growers and agricultural technicians to solve urgently. The fruit rot rate before picking in most apple producing areas is 10% to 15%, and it can reach about 30% in a major occurrence year, and even reach 50% at the highest. The annual economic loss caused by ring rot fruit disease in my country is 3 billion to 5 billion yuan, and the number of spraying in production is also increasing year by year, which not only causes huge economic losses to farmers, but also has great impact on the export and development of apples. At the same time, it also brings potential danger to environmental protection. Therefore, it is particularly important to take reasona...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/125C12R1/08
Inventor 王琦付学池梅汝鸿
Owner CHINA AGRI UNIV
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