Once-seedling forming quick propagating method for sugarcane tissue culture

Abstract

The invention relates to a method for direct regenerating of seedlings through sugarcane tissue culture. The method is as follows: young heart leaves or accretion borers at stem tips of strong sugarcane are taken as explants, and are subjected to direct regeneration of seedlings for about 40 to 50 days to obtain the seedlings with a small number of sugarcane roots; the seedlings with the small number of the sugarcane roots are transferred to a rooting medium for root inducing treatment for 10 to 20 days and then enter hardening-seedlings and provisional planting stages; or the young heart leaves or accretion borers at the stem tips of the strong sugarcane are cultured in a medium of direct regeneration of seedlings for about 50 to 70 days to obtain full seedlings of the sugarcane with good root growth, and the full seedlings directly enter the hardening-seedlings and provisional planting stages. The method does not change or only changes the culture medium for one time, effectively reduces pollution caused by frequent transfer in the process of common sugarcane lateral buds or young leaves inducing callus and differentiation, simplifies the production procedures of sugarcane tissue-culture seedlings, saves cost, has high stocking per cent, obviously shortens the production cycle of the sugarcane tissue-culture seedlings, and is suitable for factory fast propagation of sugarcane seedlings.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and more specifically relates to a method for rapid propagation of sugarcane tissue culture once seedlings are formed. Background technique [0002] Sugarcane is a vegetatively propagated crop. It has been propagated by stems and buds in production. The amount of seeds used per hectare reaches 7-10 tons. When half-stem seedlings are used in growing fields, the annual reproduction multiple is only 5-8 times. The method of double propagation in a year is adopted. The reproduction multiple is generally below 30 times. The low propagation coefficient of seedlings and the large amount of seedlings per unit area lead to slow promotion of new fine varieties and difficulty in updating varieties. After many years of continuous planting, sugarcane is often attacked by diseases, which deteriorates the quality and yield, and also accelerates the degradation of varieties. [0003] Sugarcane tis...

AI Technical Summary

Problems solved by technology

The traditional sugarcane tissue culture rapid propagation method requires many subcultures for callus induction and differentiation, high mutation rate, cumbersome operation, and high probability of contamination; sugarcane axillary bud culture and shoot tip growth cone culture can effectively reduce the mutation rate, but It needs multiple subcultures such as induction of seedlings and clustered seedlings, proliferation culture and rooting culture

The above two ways usually need to cultivate the material for at least 4 months before they can differentiate and emerge, which takes a long time and is inefficient