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Composite gene chip for food-borne pathogenic bacteria detection

A technology of food-borne pathogenic bacteria and gene chip, which is applied in the detection/testing of microorganisms, resistance to vector-borne diseases, biochemical equipment and methods, etc. It reflects the characteristics of high-throughput and high-information content of the gene chip, etc.

Inactive Publication Date: 2011-02-02
广东省疾病预防控制中心
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Problems solved by technology

The food-borne pathogen detection chips reported so far either detect a single strain of bacteria, or detect a single gene, or the purpose of the detection chip is not clear, and none of them reflect the characteristics of high throughput and high information content of the gene chip; moreover, These studies have no obvious personality and characteristics, and basically select the same or similar genes as the target genes for detection

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  • Composite gene chip for food-borne pathogenic bacteria detection
  • Composite gene chip for food-borne pathogenic bacteria detection
  • Composite gene chip for food-borne pathogenic bacteria detection

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Embodiment Construction

[0050] 1. Determination of target strains for gene chip detection

[0051] The present invention intends to select 8 kinds of food-borne pathogens that are relatively common in Guangdong Province from the above-mentioned strains for the development of detection gene chips. Vibrio haemolyticus (VP), Salmonella (Sal), Shigella (Shi), Staphylococcus aureus (SA), Campylobacter jejuni (CJ) and Escherichia coli (EC).

[0052] Sample source: All bacterial strains are from the germ bank of Guangdong Provincial Center for Disease Control and Prevention.

[0053] Hereinafter, the oligonucleotide detection gene chip developed for these 8 food-borne pathogens will be named "Biosafood-8 chip".

[0054] 2. Design of Oligo probe

[0055] A total of 351 probes were designed for 89 detection target genes of 8 food-borne pathogenic bacteria, including 1 positive control probe and 3 negative control probes (Table 4-1). The gene chip detection probes designed for 8 kinds of food-borne pathogenic bacteria...

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Abstract

The invention relates to a composite gene chip for detecting food pathogenic microbes, which comprises eight parallel sub-arrays constructed by detection probe groups for detecting each microbe in the eight food pathogenic microbes, blank lines are respectively arranged among the eight sub-arrays to be as intervals, thus constructing a big array; the eight food pathogenic microbes are listeria monocytogenes, comma bacillus, vibrio parahaemolyticus, salmonella, shigella, staphylococcus aureus, campylobacter jejuni and escherichia coli. The gene chip can clearly and accurately detect the species of eight target food pathogenic microbes, and also can definitely exclude other off-target microbe samples out of the eight target food pathogenic microbes. Each food pathogenic microbe detection sub-array on the chip can clearly and definitely distinguish off-target microbe samples from target microbe samples.

Description

Technical field [0001] The invention belongs to the field of food safety and relates to a composite gene chip for detecting food-borne pathogenic bacteria. Background technique [0002] Food poisoning refers to non-infectious (not infectious diseases) acute and subacute diseases that occur after ingesting food containing biological or chemical toxic and harmful substances or taking toxic and harmful substances as food. According to pathogenic substances, food poisoning can be divided into five categories: bacterial, chemical, animal, plant, and fungal. Among them, bacterial and chemical food poisoning are the most common. Bacterial food poisoning refers to a disease with acute gastroenteritis as the main poisoning symptom caused by eating food contaminated by pathogens or their toxins. It is the most common type of food poisoning. Common pathogens that cause bacterial food poisoning are: Vibrio parahaemolyticus, Salmonella, Staphylococcus, Escherichia coli, Vibrio cholerae, List...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12Q1/14C12Q1/10C12Q1/04
CPCY02A50/30
Inventor 王洪敏何冬梅邓小玲柯昌文杨杏芳
Owner 广东省疾病预防控制中心
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