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Culture method of phlebopus portentosus liquid bacterial

A technology of dark brown net stalk and liquid strain, applied in the biological field, can solve the problems that wild boletus resources cannot meet people's needs and cannot be artificially cultivated on a large scale, and achieve the effects of uniform size, golden color and simple cultivation method.

Active Publication Date: 2011-04-13
JINGHONG HONGZHEN AGRI TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, because the fruiting bodies of Dictyostelium pheasantii cannot be artificially cultivated on a large scale, the resources of wild boletus cannot meet people's needs, and the liquid fermentation culture of mycelium is the best way to solve this problem

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A, cultivating Dictyostelium bruceae sporocarp tissue block with strain culture medium to prepare female species, the preparation of female species medium is formulated by following raw materials and methods:

[0034] Raw material: 200g of potato, 20g of sucrose, 2g of ammonium tartrate, MgSO 4 1g, KH 2 PO 4 1g, 15g agar, 1000ml water

[0035] Preparation:

[0036] a1) Wash the fresh potatoes, peel them, weigh them, cut them into thin slices, put them in a large beaker, add a certain amount of water, boil for 20 minutes and filter to get the filtrate, add sucrose, agar, ammonium tartrate, MgSO 4 、KH 2 PO 4 , boil until the agar is completely melted, and the pH value is 6.0, to obtain the mother seed medium; divide the medium into Erlenmeyer flasks, sterilize at 120°C for 30 minutes, pour it into a petri dish in an ultra-clean workbench after cooling, and then use it ;

[0037] a2) Aseptically operate in an ultra-clean workbench, separate fruiting body tissue piec...

Embodiment 2

[0045] A, cultivating Dictyostelium bruceae sporocarp tissue block with strain culture medium to prepare female species, the preparation of female species medium is formulated by following raw materials and methods:

[0046] Raw materials: 150g of potato, 15g of sucrose, 1g of ammonium tartrate, MgSO 4 2g, KH 2 PO 4 2g, 20g agar, 1000ml water;

[0047] The following steps are the same as in Example 1.

[0048] B, fermenting and cultivating liquid bacterial classification with liquid medium, the raw material composition of liquid medium is as follows: potato 150g, sucrose 15g, ammonium tartrate 1g, MgSO 4 2g, KH 2 PO 4 2g, water 1000ml;

[0049] The following steps are the same as in Example 1.

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PUM

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Abstract

The invention provides a method for culturing Phlebopus portentosus fluid spawn. The method is characterized by comprising the following steps: culturing a strain culture medium and a fluid spawn culture medium by using potato, sucrose, agar, ammonium tartrate, MgSO4, KH2PO4 water and other raw materials, according to certain proportion; culturing an in-vitro part of Phlebopus portentosus entity to prepare strains; and carrying out fermentation culture by a fluid nutrient medium, according to a liquid culture method. In the method, the provided culture media are particularly applicable to preparation of the strains and fermentation culture of mycelium. With the method, the fermentation culture of the Phlebopus portentosus mycelium can be carried out quickly.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for cultivating liquid strains of Dictyostelium bruceae (produced in the tropical area of ​​Yunnan). Background technique [0002] Phlebopus portentosus is a delicious edible fungus with rich nutritional value and loved by consumers in the place of origin. Still can't cultivate artificially at present, and the fruiting body of the pheasant stalk boletus sold on the market all adopts wild natural growth. Liquid fermentation is the basic method for scale-up production of microbial products. It is not affected or limited by environmental and climatic conditions, and a large amount of biological products can be obtained in a short period of time. The process is easy to scale up, and has good repeatability and safety. [0003] At present, because the fruiting bodies of Dictyostelium pheasantii cannot be artificially cultivated on a large scale, the resources of wild boletus cann...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12R1/645
Inventor 纪开萍何明霞王文兵张春霞刘静刘昌芬曹旸
Owner JINGHONG HONGZHEN AGRI TECH CO LTD
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