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Plants having improved growth characteristics and methods for making the same

A technology for plants and plant cells, applied in the field of molecular biology, can solve the problems that monocotyledonous plants and plants without biomass increase cannot be used so easily.

Inactive Publication Date: 2009-02-11
CROPDESIGN NV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0040] Contrary to these teachings, it has now been found that whether expression is driven by a constitutive promoter or in a tissue-specific manner, polynucleotides encoding AT-hook transcription factors comprising the DUF296 domain, which include members of clade G1073, are found in monads Expression in leaf plants all resulted in plants with little or no increase in biomass compared to suitable control plants
This suggests that the teachings about expressing such transcription factors in dicots may not be so readily applicable to monocots

Method used

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  • Plants having improved growth characteristics and methods for making the same
  • Plants having improved growth characteristics and methods for making the same
  • Plants having improved growth characteristics and methods for making the same

Examples

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Embodiment

[0764] The invention will now be described with reference to the following examples for illustrative purposes only. The following examples are not intended to completely determine or otherwise limit the scope of the present invention.

[0765] DNA manipulation

[0766]Unless otherwise indicated, according to (Sambrook (2001) Molecular Cloning: a laboratory manual, 3rd edition, Cold Spring Harbor Laboratory Press, CSH, New York) or Ausubel et al. (1994), Current Protocols in Molecular Biology, Current Protocols ( http: / / www.4ulr.com / products / currentprotocols / index.html) 1 and 2 The standard method described in the volume carries out recombinant DNA technology. Standard materials and methods for plant molecular manipulation are described in Plant Molecular Biology Labfax (1993) edited by R.D.D. Croy published by BIOS Scientific Publications Ltd (UK) and Blackwell Scientific Publications (UK).

[0767] Statistical analysis

[0768] A two-way ANOVA (analysis of variance) corrected f...

Embodiment A

[0770] Example A: SYR

Embodiment 1

[0771] Example 1: Identification of sequences related to SEQ ID NO: 1 and SEQ ID NO: 2

[0772] Using database search tools such as Basic Local Alignment Tool (BLAST) (Altschul et al. (1990) J. Mol. Biol. 215: 403-410; and Altschul et al. (1997) Nucleic Acids Res. 25: 3389-3402), in the sequence maintained by the Entrez Nucleotide Database of the National Center for Biotechnology Information (NCBI), identify the sequence related to SEQ ID NO:1 (full-length cDNA, EST or genomic sequence) and / or the sequence related to SEQ ID NO: 2 related protein sequence. Generally, BLAST programs are used to find regions of local similarity between sequences by comparing nucleic acid or polypeptide sequences with sequence databases, and by calculating the statistical significance of matches. Apply the TBLASTN algorithm to the polypeptide encoded by nucleic acid SEQ ID NO:1, use the default settings, and turn on the filter to ignore low-complexity sequences. The output window of the analysis is a ...

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Abstract

The present invention relates generally to the field of molecular biology and concerns a method for improving various plant growth characteristics by modulating expression in a plant of a nucleic acid encoding a GRP (Growth-Related Protein). The present invention also concerns plants having modulated expression of a nucleic acid encoding a GRP, which plants have improved growth characteristics relative to corresponding wild type plants or other control plants. The invention also provides constructs useful in the methods of the invention. The GRP may be one of the following: Seed Yield Regulator (SYR), FG-GAP1 CYP90B, CDC27, AT-hook transcription factors, DOF transcription factors and Cyclin Dependent Kinase Inhibitors (CKIs).

Description

Technical field [0001] The present invention generally relates to the field of molecular biology and relates to methods for improving various plant growth characteristics by modulating the expression of a nucleic acid encoding GRP (growth-related protein) in plants. The present invention also relates to plants with regulated expression of GRP encoding nucleic acid, which plants have improved growth characteristics compared to corresponding wild-type plants or other control plants. The invention also provides constructs used in the methods of the invention. Background technique [0002] In view of the continuous growth of the world's population, the area of ​​land available for agriculture has been declining. Improving agricultural efficiency and increasing the diversity of horticultural plants are still the main goals of research. Conventional methods for crop and horticultural plant improvement utilize selective breeding techniques to identify plants with desired characteristics...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/10C12N15/82C07K14/415
CPCC12N15/8261C12N15/8262C07K14/415Y02A40/146
Inventor V·弗兰卡德C·勒佐A·I·桑兹莫林纳罗C·达曼
Owner CROPDESIGN NV
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