Neutrophilic granulocyte activity detection method

Abstract

A neutrophil activity test method is that 1 weight portion of pyogenic bacteria inactivated vaccine is mixed evenly with 1 to 1.5 portions of medical turpentine, 0.3 to 0.5ml of the reagent is dropped in carrier medicinal cotton to be pasted on the one-inch skin of the left hand or the right hand of a human body by using an adhesive plaster, the adhesive plaster is ripped off after 12 to 24 hours of the action time of the reagent, vacuole is formed on the skin, the position of the vacuole is pasted with an adhesive plaster with indicators for 24 to 25 hours, then the adhesive plaster is uncovered to observe the fading situations. The test method is that acute suppurative inflammation is produced on the skin of the human body through an induced inflammatory method to observe the skin suppuration, detect and undergo the activity of myeloperoxidase (MPO) to judge whether the functional neutrophil (PMN) activity has defects and obstacles. The neutrophil activity test method has accuracy of more than 92 percent through the tests of 25 patients with various cancers, 23 of the 25 patients having no fading of the indicators and 2 of the 25 patients having unobvious fading reaction of the indicators, and the tests on 40 healthful human bodies with complete fading reaction of the indicators.

Description

technical field [0001] The invention relates to a method for detecting neutrophil activity. Background technique [0002] Neutrophils (PMN) are an important part of the body's defense system and are related to the body's non-specific anti-infection. This type of cell has a powerful phagocytosis and sterilization function. In recent years, studies have found that neutrophils also have a high activity of killing cancer cells. The process of neutrophil action undergoes chemotactic movement, recognizes and binds to phagocytosis, and then takes it into the cell, and finally kills and degrades the pathogen. [0003] At present, neutrophils are one of the important indicators of acute inflammation. Most domestic hospital laboratories only do routine white blood cell counts, which cannot reflect the activity and complex process of neutrophils killing pathogens. The existing neutrophil function testing is not only complicated in process, but also requires high technical requirement...

AI Technical Summary

Problems solved by technology

[0003] At present, neutrophils are one of the important indicators of acute inflammation. Most domestic hospital laboratories only do routine white blood cell counts, which cannot reflect the activity and complex process of neutrophils killing pathogens.

The existing neutrophil function test is not only complicated in process, but also requires high technical requirements, which is not easy to popularize and popularize, and is not conducive to the general screening and screening of immune function deficiencies in the population, including cancer.