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Construction method of micro/nano graph of DNA molecule

A DNA molecule and construction method technology, applied in the field of micro/nano pattern construction, can solve the problems of inability to avoid DNA molecule crossover phenomenon, low efficiency, etc., and achieve the effect of low cost and simple operation means

Inactive Publication Date: 2008-12-17
SHANGHAI INST OF APPLIED PHYSICS - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the first two methods belong to a category that can only work on DNA molecules with single-stranded DNA ends or designed single-stranded DNA. At the same time, the DNA graph constructed by this method cannot avoid the crossover phenomenon of DNA molecules.
Although the method (3) can be used to construct more complex DNA graphics, the efficiency is very low; the method (4) can often only construct some simple DNA molecular graphics

Method used

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  • Construction method of micro/nano graph of DNA molecule
  • Construction method of micro/nano graph of DNA molecule
  • Construction method of micro/nano graph of DNA molecule

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] In order to demonstrate the change process and monitor the regularity of micro / nano pattern formation, this embodiment performs liquid disturbance step by step.

[0024] First, stick the newly stripped HOPG [Veeco Precision Instrument Co., Ltd., USA] on the rotating shaft of the centrifuge, and then take 10 microliters of a DNA solution (TE buffer, pH 8.0) with a concentration of 10 ng / μl and drop it on the At the center of the HOPG surface of the substrate, after standing for 3 to 5 minutes, the DNA solution was blown off with a suction ear ball, and the AFM observation showed that the DNA molecules were randomly adsorbed on the substrate, as shown in figure 1 shown in A. Then take 10 microliters of double-distilled water drop at the center of the HOPG surface, and start the centrifuge to rotate at 1200 rpm, and after 3 minutes, increase the speed to 2000 rpm and rotate for 5 seconds (S) to spin the water droplet AFM observation shows that the DNA molecules are ordere...

Embodiment 2

[0026] Take 100 μl of DNA solution with a concentration of 2ng / μl and drop it on the center of the surface of the substrate HOPG, let it stand for 10 minutes, and then use filter paper to absorb the DNA solution. Then take 100 microliters of double-distilled water droplets on the center of the HOPG surface, and start the centrifuge to rotate at 600 rpm. After 10 minutes, increase the rotation speed to 2000 rpm and rotate for 5 seconds to spin off the water droplets. image 3 The regular and complex micro / nano (size between 50nm and 800nm) patterns of DNA molecules are shown.

Embodiment 3

[0028] Take 5 microliters of DNA solution with a concentration of 20ng / μl and drop it on the center of the HOPG surface of the substrate, let it stand for 1 minute, start the centrifuge to rotate at 1400 rpm, and increase the rotational speed to 2000 rpm after 3 minutes. Spin the water droplet away, we can get as Figure 4 The regular and complex micro / nano (size between 50nm and 800nm) patterns of DNA molecules are shown.

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Abstract

The invention discloses a construction method of the micron / nano pattern of DNA molecules, which comprises the steps of: dispersing and adsorbing the DNA molecules on a highly-oriented pyrolytic graphite substrate, adopting liquid disturbance to regularly assign the DNA molecules into a nano or micron pattern. The method of the invention can construct the complicated micron / nano pattern of the DNA molecules efficiently, has simple operational approaches and low cost. The constructed nano pattern of the DNA molecules can provide a good template for designing controllable reaction on the basis of the nano devices and the nano level of the DNA molecules.

Description

technical field [0001] The invention relates to a method for constructing micro / nano patterns of DNA molecules. Background technique [0002] DNA molecule is an important genetic material in living organisms. It is a uniform double helix molecule with a diameter of about 2 nanometers formed by complementary bases, and has relatively stable physical and chemical properties. Due to these unique properties of DNA molecules, with the development of nanotechnology, people realize that DNA molecules are a kind of excellent nanomaterials with broad prospects. Nano-patterns constructed using DNA molecules can provide good templates for the design of nano-devices based on DNA molecules. The "reaction chamber" controls some biological and chemical reactions in various "intervals"; at the same time, it is also possible to study the "size" effect of some substances in these nanoscale "reaction chambers". Therefore, the DNA molecule construction Nano-patterns have been highly valued b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/00
Inventor 王化斌胡钧张益孙洁林王鹏
Owner SHANGHAI INST OF APPLIED PHYSICS - CHINESE ACAD OF SCI
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