Method for extracting stem-possessing plant leaf fibrovascular bundle juice

A technology of plant leaves and vascular bundles, which is applied in the field of botany, can solve problems such as easy pollution errors, and achieve the effects of large sample size, wide application range, and accurate test results

Inactive Publication Date: 2010-06-30
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to overcome the limitations of the existing methods for extracting plant vascular bundle juice, the shortcomings of easy pollution and large errors, the present invention provides a method for directly obtaining the vascular bundle juice of stalked plant leaves by the pressure method, which effectively solves the problem

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Select a few leaves of field cotton, cut them off with a blade at the base of the petiole, put them in a porcelain plate, use wet gauze to keep them moist, and wrap them in black cloth to avoid light.

[0017] Use a clean and sharp blade to circumcise along the outer edge of the leaf at 0.5-1cm to open the leaf vascular bundle; keep the petiole at 3-8cm, and cut off the excess petiole with a blade; cut at a distance of 0.5-1cm from the end of the petiole. Out of the screen part. Put the treated cotton leaves into the pressure chamber, keep the petiole outside the pressure chamber, pressurize, make the juice in the leaf screen tube flow out from the petiole incision, and collect the juice flowing out from the screen tube incision. The extracted solution was stored in a refrigerator at -37°C until testing. The working gas in the pressure chamber is nitrogen in steel cylinders, and the working pressure is 1.0-5kPa.

[0018] Analysis and comparison of soluble sugar conten...

Embodiment 2

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PUM

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Abstract

The invention relates to the botany field, in particular to a method for extracting juice in a vascular bundle of a plant leaf with stems, comprising the following steps of: circularly cutting along 0.5 to 1cm of the outer margin of the leaf by a clean and sharp blade to open the vascular bundle of the leaf margin; retaining 3 to 8cm of the stem, and removing the superfluous stems by the blade; semi-circularly cutting the position which is between 0.5 and 1cm from the end part of the stem, and removing the sieve vessel part; putting the processed plant leaf into a depression box, leaving the stem outside the depression box, pressurizing the depression box to enable juice in the sieve vessel of the leaf to flow out from a cut of the stem, and collecting the juice flowing out from the cut of the sieve vessel. The method has the advantages of wide application range, large acquisition of samples, no pollution of samples and more accurate test result.

Description

technical field [0001] The invention relates to the field of botany, in particular to a method for extracting vascular bundle juice from leaves of stalked plants. Background technique [0002] Piercing-sucking mouthpart pests use their stylets to insert into the vascular bundles of the host plant sieve tubes to suck juice to obtain nutrients (Gerling, D. and M. Lindenbaum. Host-plant related behavior of Bemisia tabaci. WPRSBull., 1991, 14: 83~88.) (Liu Guangjie, Wilkins R M, Saxena R C. Utilization of carbohydrates in different insect-resistant rice plants by white-backed planthoppers. Acta Entomology, 1995, 38(4): 421-427), therefore, in order to accurately To analyze the relationship between host nutrition and piercing-sucking mouthparts, it is necessary to accurately obtain plant vascular juice. [0003] The traditional method of extracting plant vascular bundle juice mainly uses the "aphid kissing needle method", that is, using an aphid to puncture the plant leaf, and t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/28
Inventor 周福才
Owner YANGZHOU UNIV
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