Low-expression piRNA probe for detecting gastric tissue and detection process thereof

A low-expression, tissue-based technology, applied in the field of piRNA, can solve problems that do not involve gastric tissue

Inactive Publication Date: 2008-10-22
NINGBO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] my country is a high-incidence area of ​​gastric cancer, and none of the existing t...

Method used

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  • Low-expression piRNA probe for detecting gastric tissue and detection process thereof
  • Low-expression piRNA probe for detecting gastric tissue and detection process thereof
  • Low-expression piRNA probe for detecting gastric tissue and detection process thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A low-expression piRNA probe for gastric tissue is a microfluidic chip synthesized in situ by a photosensitive reagent. The sequence of the probe consists of a chemically modified oligonucleotide coding fragment and an extension arm fragment, in which the oligonucleotide The nucleotide coding segment is hsa_piR_000370 sequence.

Embodiment 2

[0028] A low-expression piRNA probe for gastric tissue is basically the same as in Example 1, except that the oligonucleotide coding fragment is the hsa_piR_009567 sequence.

Embodiment 3

[0030] A low-expression piRNA probe for gastric tissue is basically the same as in Example 1, except that the oligonucleotide coding fragment consists of hsa_piR_000370 sequence and hsa_piR_009567 sequence.

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Abstract

The invention discloses a low expression piRNA probe for stomach tissue. The sequence of the probe consists of a chemically modified oligonucleotide coding fragment and an extendible arm fragment, wherein the oligonucleotide coding fragment is at least one of the following piRNA sequences: hsa_piR_000370, hsa_piR_009567, hsa_piR_000216, hsa_piR_000728, hsa_piR_010229, hsa_piR_000990, hsa_piR_000460, hsa_piR_006105, hsa_piR_005837, hsa_piR_007743, hsa_piR_009884, hsa_piR_000001 and hsa_piR_004611. The probe can carry out the hybridization detection of piRNAs of the stomach tissue and detect the piRNAs with abnormally low expression in the stomach tissue. As diagnosis markers of canceration of the stomach tissue, the piRNAs can further serve as targets of designing directional stomach cancer drugs. Therefore, the probe provides the useful reference for the diagnosis and directional treatment of stomach cancer.

Description

technical field [0001] The invention relates to piRNA, in particular to a low-expression piRNA probe for detecting gastric tissue and a detection method thereof. Background technique [0002] In addition to protein-coding genes in eukaryotic genome, a large part of DNA is transcribed into non-coding RNAs (noncoding RNAs, ncRNAs). Recent studies have found that many ncRNAs can regulate gene expression at multiple levels, especially the role of small RNAs in the regulation of gene expression and its relationship with the occurrence and development of diseases has attracted more and more attention. Previous studies have discovered two types of small RNAs that play an important role in regulating gene expression—short interfering RNA (short interfering RNA, siRNA) and microRNA (microRNA, miRNA). [0003] In 2006, four laboratories in Russia, Japan and the United States reported almost simultaneously that there was another type of small RNA in vivo besides siRNA and miRNA. The ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
Inventor 郭俊明肖丙秀蒋振
Owner NINGBO UNIV
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