Microcurrent controlled capillary tube electrophoresis liquid core waveguide fluorescence testing apparatus

Abstract

A micro flow control capillary electrophoresis liquid core waveguide fluorescence detector includes a liquid core waveguide capillary, a laser light source, a photodetector, a high-voltage power supply, an electrode, a liquid storage container, a sample tube, a pinhole diaphragm, a filter and a shadow shield; the device makes use of a liquid core waveguide phenomenon and uses the same liquid corewaveguide capillary as a capillary electrophoresis separation channel and for fluorescence signal transduction. In the invention, the light given out by the laser light source can be not focused by alens but directly radiates on the liquid core waveguide capillary through the pinhole diaphragm; the fluorescence led out by the outlet port of the liquid core waveguide capillary can not be collectedbythe lens but directly enters into the photodetector through the filter. The liquid core waveguide capillary is taken as a micro flow control capillary electrophoresis separation channel and an optica l conduction channel, so as to furthest reduce optical elements, simplify an optical system, make the structure more compact, so as to provide a feasible technical route for developing miniature microflow control analytical instruments.

Description

technical field [0001] The invention belongs to the technical field of microfluidic analysis, in particular to a microfluidic capillary electrophoresis liquid core waveguide fluorescence detection device. Background technique [0002] Microfluidics (or microfluidics) is the technology and science of manipulating nanoliter (n1) to picoliter (p1) volume fluids in micron-scale structures [Fang Zhaolun, Fabrication and Application of Microfluidic Analysis Chip, Chemistry Industrial Press, 2005, 4]. Microfluidic analysis with microfluidics as the core technology has become the forefront of the development of contemporary analytical science. Microfluidic analysis has obvious advantages such as fast analysis speed, less consumption of samples and reagents, high degree of integration, and small volume, and has gradually played an important role in chemical and biological testing. [0003] The miniaturization of the analysis system puts forward corresponding requirements for the de...

AI Technical Summary

Problems solved by technology

However, there are still few applications in simplifying the optical system and miniaturizing microfluidic analysis instruments.

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