Bacillus foecalis alkaligenes and method for catalysis degradation of phynol wastewater
A technology of Alcaligenes faecalis and catalytic degradation, which is applied in the directions of microorganism-based methods, chemical instruments and methods, biochemical equipment and methods, etc., can solve the problem of unsatisfactory treatment effect of phenol-containing wastewater, low microbial concentration and large floor space and other problems, to achieve the effect of good catalytic degradation effect, high microbial concentration and not easy to run off
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Embodiment 1
[0016] The Alcaligenes faecalis JH 1013 strain was cultured in the beef extract peptone culture solution for 24 hours, the shaking speed was 100 rpm, the temperature was 35°C, and the pH was 7. centrifuge, take the centrifuge and dissolve in phosphate buffer solution with pH 8.0 to obtain a bacterial suspension containing Alcaligenes faecalis, and absorb the Alcaligenes faecalis with a calcium carbonate carrier to obtain immobilized Alcaligenes faecalis. Add the immobilized Alcaligenes faecalis into the phenol wastewater, and the phenol concentration in the phenol wastewater is 1000mg·L -1 , the amount of immobilized Alcaligenes faecalis is 0.5wt% of the weight of phenol wastewater, the shaking rate is 200rpm, the temperature is 32°C, the pH is 8, the time is 18h, and the degradation rate is 99.4%.
Embodiment 2
[0018] The Alcaligenes faecalis JH 1013 strain was cultured in the beef extract peptone culture solution for 24 hours, the shaking speed was 150 rpm, the temperature was 30°C, and the pH was 8. After centrifugal separation, the centrifuge was taken and dissolved in phosphate buffer solution with pH 7.2 to obtain a solution containing Alcaligenes faecalis, and the Alcaligenes faecalis was adsorbed by Mg-Al hydrotalcite carrier to obtain immobilized Alcaligenes faecalis. Add the immobilized Alcaligenes faecalis into the phenol wastewater, and the phenol concentration in the phenol wastewater is 1800mg·L -1 , the amount of immobilized Alcaligenes faecalis is 1.5wt% of the weight of phenol wastewater, the shaking speed is 300rpm, the temperature is 28°C, the pH is 7, the time is 36h, and the degradation rate is 82.9%.
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