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Method for analyzing rice blast resisting gene

A technology of rice blast resistance gene and disease resistance gene, which is applied in the fields of biochemical equipment and methods, determination/inspection of microorganisms, etc.

Inactive Publication Date: 2008-08-13
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This is a very heavy work, and it is difficult to analyze the relationship between different disease resistance genes

Method used

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  • Method for analyzing rice blast resisting gene
  • Method for analyzing rice blast resisting gene
  • Method for analyzing rice blast resisting gene

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] 1 Using the method mediated by Agrobacterium, transform the widely non-pathogenic strains to obtain CY2, and obtain a large number of transformants;

[0016] 2 Inoculate the obtained transformants on the rice near-isogenic lines of known disease-resistant genes, obtain monospore strains from susceptible disease spots, and connect them back to the near-isogenic lines to confirm the pathogenicity, and obtain 3 The strains T1(VirPi-ta) and T2(VirPi-ta) with mutations in pathogenicity 2 ) and T3 (VirPi-a);

[0017] 3 Sow the rice variety to be tested and three corresponding near-isogenic lines (A, B, C) with known disease-resistant genes in different holes in the seedling tray, and use Lijiang Xintuan Heigu as a susceptible control, and use conventional methods Manage and wait for the seedlings to grow to 3 leaves and 1 heart;

[0018] 4 Cultivate the three strains and the wild-type strain CY2 on oat medium, and prepare spore suspension after sporulation;

[0019] 5. Ino...

Embodiment 2

[0025] 1 Using the method mediated by Agrobacterium, transform the widely non-pathogenic strains to obtain CY2, and obtain a large number of transformants;

[0026] 2. Inoculate the obtained transformants on the rice near-isogenic lines of known disease-resistant genes, obtain monospore strains from susceptible disease spots, and connect them back to the near-isogenic lines to confirm the pathogenicity, and obtain 4 The strains T1 (VirPi-5, Pi-7), T2 (VirPi-7), T3 (VirPi-5, Pi-9) and T4 (VirPi-7, Pi-12) with pathogenic mutations.

[0027] 3 Sow the rice variety to be tested and 4 corresponding near-isogenic lines of known disease-resistant genes in different holes in the seedling tray, and use Lijiang Xintuan Heigu as a susceptible control, manage with conventional methods, and wait until the seedlings grow to 3 leaves and 1 heart are spare;

[0028] 4 respectively culture the 4 bacterial strains and the wild-type strain CY2 on the oat medium, and prepare the spore suspension...

Embodiment 3

[0035] 1 Using the method mediated by Agrobacterium, transform the widely non-pathogenic strains to obtain CY2, and obtain a large number of transformants;

[0036] 2. Inoculate the obtained transformants on rice near isogenic lines with known disease-resistant genes, obtain monospore strains from susceptible lesions, and connect them back to the corresponding lines to confirm the pathogenicity, and obtained 8 The pathogenic strains T1 (VirPi-a, Pi-i), T2 (VirPi-i), T3 (VirPi-K s ), T4 (VirPi-K P , Pi-k H ), T5 (VirPi-k H ), T6 (VirPi-Z, Pi-ta), T7 (VirPi-ta), T8 (VirPi-b);

[0037] 3 Sow the rice variety to be tested and 8 corresponding near-isogenic lines of known disease-resistant genes in different holes in the seedling tray, and use Lijiang Xintuan Black Valley as a susceptible control, manage with conventional methods, and wait until the seedlings grow to 3 leaves and 1 heart are spare;

[0038] 4 Cultivate the 8 strains and the wild-type strain CY2 on the oat mediu...

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Abstract

The invention relates to a method for analyzing rice blast resistance gene. The technical scheme of the invention is: 1)searching a property-stable avirulent strain; 2)conversing the strain by agrobacterium-mediated method, inoculating the transformant on near-isogenic lines of rice, getting single-ascospore strain having mutated pathogenicity from susceptible type spot; and 3)return inoculating, identifying and confirming the pathogenicity of the single-ascospore strain, and getting a set of near strains for identifying resistance gene of varieties. The invention can construct a set of rice blast resistance near-isogenic gene stains to deduce resistance gene, directly inoculate stable variety by mutant without using resistance after rice hybridization and inoculation hybridization, as well as allelism test, find out the resistance gene of variety according to the resistance type; and can be used for deducing resistance gene of rice variety containing single or multiple resistance genes.

Description

Technical field: [0001] The invention relates to a method for analyzing the rice blast resistance gene by using the rice blast fungus near and other strains, which belongs to the field of plant protection. Background technique: [0002] Rice blast is a worldwide disease. Many years of practice have proved that cultivating and planting disease-resistant varieties is the safest, economical and effective control measure for this disease. However, rice blast fungus is a pathogenic group that is more prone to mutation, and often causes a newly promoted variety to lose resistance after 3 to 5 years of planting. In order to achieve stable and high yields, breeders have to use new disease-resistant genes or gene combinations to breed new disease-resistant varieties. Therefore, accurate and reliable analysis of rice blast resistance genes is of great significance for rice disease-resistant breeding. So far, more than 40 rice blast resistance genes have been identified. However, the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12Q1/02
Inventor 何月秋周惠萍吴毅馨
Owner YUNNAN AGRICULTURAL UNIVERSITY
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