Stream type cell detection device and its accomplished stream type cell detection method

Abstract

The invention discloses a flowing type cell testing device and a method of realizing the flowing type cell test thereof. The flowing type cell testing device provided by the invention comprises a flowing chamber, a sample injector, a reaction bath, a sheath liquid bath and a plurality of valves, wherein the reaction bath is connected with a main interface of the sample injector through a first valve and a sixth valve, a seventh valve arranged between the sixth valve and the first valve is connected with the sheath liquid bath or other pressure storage baths. The flowing type cell testing method comprises the processes of establishing and testing a stable sample flow, etc. In the process of establishing and testing the stable sample flow, the sixth valve is closed and simultaneously the first valve and the seventh valve are opened, thereby enabling the sheath liquid in the sheath liquid bath to flow into the reaction bath for cleaning the reaction bath from bottom to top. The flowing type cell testing device and the method of realizing the flowing type cell test provided by the invention are capable of simultaneously cleaning the reaction bath from bottom to top in the testing process, thereby greatly improving the working efficiency of the whole system.

Description

technical field [0001] The invention relates to a flow cytometric detection device and a flow cytometric detection method realized by using the device. Background technique [0002] Current flow cytometry devices, such as mid-to-high-end hematology analyzers and flow cytometers, all use the principle of flow cytometry to count and classify cells or particles. The core of flow cytometry is the sheath flow (fluid focusing) technology in which the cells or particles of the sample to be tested pass through the detection area one by one. In the product application of this technology, due to the need for measurement speed, it is necessary to ensure that a stable sample flow can be quickly established within a limited time, and at the same time, it can also quickly and effectively clean the relevant liquid circuit components. [0003] like figure 1 As shown, the flow cytometry detection system includes a light source system for generating laser light, a fluid system, and a photoe...

AI Technical Summary

Problems solved by technology

In the prior art, when the downstream pipeline is cleaned, the pipeline between the main interface of the sample syringe and the sample preparation port of the reaction pool is not and cannot be cleaned. The sample has been cleaned, so that the residue of the sample from the last measurement may affect the measurement result of this time

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