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Novel medical stone micro-ecological repairing additive and preparation method thereof

A medical stone and micro-ecological technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., to achieve the effect of repairing the micro-ecological environment, promoting the cycle of carbon, nitrogen and oxygen, and preventing the development of eutrophication

Inactive Publication Date: 2008-07-30
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There have been some researches at home and abroad on the microecological bacteria preparations for purifying water bodies and restoring the water ecological environment. However, there is no microecological bacteria that uses medical stone as a carrier, absorbs high-density, high-activity bacterial liquid, and can be used on a large scale. Reports on bacterial preparations

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Follow these steps:

[0021] 1) Activate each slant strain stored at 4°C, and culture them in shake flasks respectively. Bacillus subtilis, Bacillus licheniformis and Bacillus megaterium were all cultured with starch medium containing 10% humic acid, cultured at 37°C for 20-24h; Streptomyces luteus was cultured with starch medium containing 10% humic acid, 32 Cultivate at ℃ for 36-42 hours.

[0022] 2) Level-1 liquid expansion culture (the three kinds of Bacillus and Streptomyces flavinus are expanded separately, and the culture medium and culture conditions are the same as above). The inoculum size is 5%.

[0023] 3) Secondary liquid expansion culture (the three kinds of bacillus are cultured in a mixed manner according to the volume ratio of 1:1:1. Streptomyces flavinus is separately expanded culture. The culture medium and culture conditions are the same as above). The inoculum size is 5%.

[0024] 4) Tertiary liquid expansion culture (mixed expansion culture of ...

Embodiment 2

[0031] Follow these steps:

[0032] 1) Activate each slant strain stored at 4°C, and culture them in shake flasks respectively. Bacillus subtilis, Bacillus licheniformis and Bacillus megaterium were cultured in starch medium containing 10% humic acid, cultured at 37°C for 24h; Streptomyces luteus was cultured in starch medium containing 10% humic acid, cultured at 32°C 42h.

[0033] 2) Level-1 liquid expansion culture (the three kinds of Bacillus and Streptomyces flavinus are expanded separately, and the culture medium and culture conditions are the same as above). Inoculum volume 10%.

[0034] 3) Secondary liquid expansion culture (the three kinds of bacillus are cultured in a mixed manner according to the volume ratio of 1:1:1. Streptomyces flavinus is separately expanded culture. The culture medium and culture conditions are the same as above). Inoculum volume 10%.

[0035] 4) Tertiary liquid expansion culture (mixed expansion culture of three kinds of bacillus. Strepto...

Embodiment 3

[0042] Follow these steps:

[0043] 1) Activate each slant strain stored at 4°C, and culture them in shake flasks respectively. Bacillus subtilis, Bacillus licheniformis and Bacillus megaterium were all cultured in starch medium containing 10% humic acid, cultured at 37°C for 22 hours; Streptomyces luteus was cultured in starch medium containing 10% humic acid, cultured at 32°C 40h.

[0044] 2) Level-1 liquid expansion culture (the three kinds of Bacillus and Streptomyces flavinus are expanded separately, and the culture medium and culture conditions are the same as above). Inoculum volume 10%.

[0045] 3) Secondary liquid expansion culture (the three kinds of bacillus are cultured in a mixed manner according to the volume ratio of 1:1:1. Streptomyces flavinus is separately expanded culture. The culture medium and culture conditions are the same as above). The inoculum size is 5%.

[0046] 4) Tertiary liquid expansion culture (mixed expansion culture of three kinds of baci...

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PUM

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Abstract

The invention relates to a preparation and the preparation method for restoring the microecological bacteria on the interface layer of water-sediment. The microecological bacteria preparation uses 0.2-0.4mm of medical stone as carrier, absorbs and fixes the composite microbe optimally combined by bacillus subtilis, bacillus licheniformis, bacillus megatherium, and thin yellow streptomyces. The amount ratio of bacillus subtilis to bacillus licheniformis to bacillus megatherium approximates to one to one to one; the volume ratio of the mixture liquid of the three kinds of bacillus to the thin yellow streptomyces liquid is two to one. The invention has the advantages that spreading the preparation of the invention into the interface layer of functional water body water-sediment in the spring March to April each year can prevent blue algae (aerugo micro-capsule algae is the most) from fulminantly growing in summer June to August. The preparation can improve the quality of water, can prevent eutrophication development, and can promote the carbon, nitrogen, and oxygen circulation on the interface layer of water-sediment. The microecological bacteria preparation has high effective colony amount and strong adaptability; and is effective to restore the microecological environment at the interface layer of water-sediment.

Description

technical field [0001] In the field of biological technology, the present invention relates to a probiotic preparation and a preparation method thereof. Background technique [0002] At present, my country's freshwater lakes, rivers and other surface waters are already in a state of eutrophication or high eutrophication, as evidenced by successive cyanobacteria outbreaks in Taihu Lake, Chaohu Lake and Dianchi Lake. To control or prevent the outbreak of blue-green algae, in addition to strictly controlling the pollution of point and non-point sources around lakes and rivers, it is a feasible and effective measure to add an appropriate amount of micro-ecological bacteria preparations at an appropriate time. There have been some researches at home and abroad on the microecological bacteria preparations for purifying water bodies and restoring the water ecological environment. However, there is no microecological bacteria that uses medical stone as a carrier, absorbs high-densit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N11/14C02F3/34C12R1/125C12R1/10C12R1/11C12R1/465
Inventor 张清敏崔合香崔小会李燕钟欢陈卫民孙红文
Owner NANKAI UNIV
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