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Human oocyte sequence vitrification refrigerating fluid and melting fluid

A technology of vitrification and human oocytes, applied to germ cells, animal cells, vertebrate cells, etc., can solve the problems of inability to obtain clinical pregnancy rate, decline in embryo quality, safety threats, etc., and achieve a good recovery survival rate , reduced toxicity, and good developmental potential

Inactive Publication Date: 2011-04-13
SHANDONG PROVINCIAL HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

This type of freezing solution mainly has the following deficiencies: 1. High concentration leads to high toxicity, which poses a threat to the safety of oocytes for human reproductive purposes after cryopreservation; 2. Embryos obtained after oocyte cryopreservation Decreased quality and unsatisfactory clinical pregnancy rates

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0018] (1) Preparation of freezing liquid and melting liquid:

[0019] Base fluid:

[0020] Add 10ml of human blood albumin to 100ml of human fallopian tube fluid containing HEPES;

[0021] Refrigerant one:

[0022] Add 1ml Ficoll 70 and 0.065mol sucrose to 9ml base solution

[0023] Refrigerant two:

[0024] Add 1ml Ficoll 70, 0.065mol sucrose, 1ml ethylene glycol and 0.075mol dimethyl sulfoxide to 8ml base solution;

[0025] Melting solution 1: add 0.01mol sucrose to 10ml base solution;

[0026] Melting solution 2: add 0.006mol sucrose to 10ml base solution;

[0027] Melting solution 3: add 0.003mol sucrose to 10ml base solution;

[0028] Solution 4: Add 0.0015mol sucrose to 10ml base solution.

[0029] (2) How to use freezing liquid and melting liquid

[0030] When freezing, put the oocytes to be cryopreserved into the base solution for 5 minutes to balance first, then put into the freezing solution 1 for 3 minutes; (Formula) Dip freezing solution 2 with a volume n...

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Abstract

The invention discloses a human oocyte sequence vitrification refrigerating fluid and thaw liquid belonging to the cell preservation field in biology, which adopts human oviduct fluid which contains HEPES and human blood protein as basic liquid and low-density Ficoll 70, sucrose, ethylene glycol and dimethyl sulphoxide as protective agent. Compared with the prior technique, the human oocyte sequence vitrification refrigerating fluid and thaw liquid of the invention is low in toxicity, good in preservation effect and the like, which can be widely applied in the vitrification preservation of human oocyte.

Description

technical field [0001] The invention relates to the field of cell preservation in biology, in particular to a human oocyte sequence vitrification liquid and thawing liquid. Background technique [0002] Vitrification technology is a preservation technology for various egg cells and embryos in the field of biology. Vitrification cryopreservation of human oocytes is an important technical means to establish a human egg bank and solve the problem of fertility preservation for the majority of infertile patients. Although there are many kinds of vitrification fluids and thawing fluids applied to human egg cells in the prior art, none of them has been recognized by everyone and can obtain a stable and good recovery effect. For example, 1.2-propanediol (PROH) and ethylene glycol (EG) are commonly used as protective agents in refrigerants, and their concentrations are as high as 15%. This type of freezing solution mainly has the following deficiencies: 1. High concentration leads ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02C12N5/075
Inventor 陈子江李媛赵跃然
Owner SHANDONG PROVINCIAL HOSPITAL
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