Salmonella, Listeria monocytogenes, bacillus coli multiple PCR rapid detection kit and uses thereof

Abstract

The invention discloses a method simply and rapidly detecting Salmonella, Listeria monocytogenes producing monocyte, and escherichia coli simultaneously and a detection reagent kit invented based on the method. Firstly the DNA templates of the three microorganisms are extracted through hot dehiscing (Salmonella, escherichia coli) and enzymolysis method (Listeria monocytogenes), then the specific primers according to salmonella, Listeria monocytogenes producing monocyte, and escherichia coli are designed respectively, the practical amplification fragments are salmonella 320bp, Listeria monocytogenes producing monocyte 440bp, and escherichia coli 706bp. The invention has the advantages of celerity and nicety compared with the bacteria separation, identification of classical method and serological typing test method, strong specificity and high sensitivity compared with the generic multiplex PCR method, and the ability to detect and identify the three pathogens simultaneously.

Description

technical field [0001] The invention belongs to the field of molecular biology detection, and discloses a multiple PCR method and a detection kit for simultaneously detecting three pathogenic bacteria of Salmonella, Listeria monocytogenes and Escherichia coli. Background technique [0002] Salmonella and Listeria monocytogenes are important zoonotic pathogens, and are the two most important foodborne pathogens listed by the World Health Organization (WHO). Salmonella can not only cause animal diseases, but also cause typhoid fever, paratyphoid fever, sepsis, gastroenteritis and food poisoning in humans. The food poisoning cases caused by this are the first or second in food poisoning. Listeria monocytogenes is a food-borne pathogen that can cause diseases such as abortion, sepsis and meningitis in animals and humans. Newborns, the elderly, pregnant women and immunocompromised patients are infected by Listeria monocytogenes high-risk groups. In developed countries, the inci...

AI Technical Summary

Problems solved by technology

The traditional national and industry standards for Salmonella and Listeria monocytogenes mostly use traditional plate culture or enzyme-linked immunoreaction methods, and it takes at least 7 to 10 days to obtain the test results, and different test procedures are used for the operation , method, and report separately, the inspection method is cumbersome, time-consuming and labor-intensive, and has low specificity, which brings great inconvenience to the import and export of food

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