Bacterium agent for renovation of organic pollution aquifer, producing and using method of the same
A technology of organic pollution and bacterial agent, applied in the field of microorganisms, can solve the problems of nitrobenzene polluted water, slow degradation of environmental pollutants, unable to continue to degrade pollutants, etc. Strong, continuous enhancement of the effect of water purification
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Embodiment 1
[0022] The bacterial agent is composed of aerobic microorganisms: Bacillus, Arthrobacter, Aeromonas, Alealigenes, and Pseudomonas Bacillus (Pseudomonas), the parts by weight are 1:1:2:4:10, and the balance is the culture solution containing nitrobenzene.
[0023] Specifically: Bacillus megaterium, Arthrobacter simplex, Aeromonas hydrophila, Alealigenes eutrophus and Pseudomonas fluorescens.
[0024] The preparation method of bacterium agent: the aerobic microorganism that is 10% by weight percentage is inoculated in the Erlenmeyer flask that 150ml sterilized culture solution is housed, and the initial concentration of nitrobenzene reaches 20mg / L in the culture medium, and gauze pad is sealed, and is placed in 28 Shake culture in a constant temperature shaking incubator at ℃ for 5 days, then take out 1 / 3 of the culture solution, and then add the same amount of culture solution to make the initial concentration of nitrobenzene in the medium reach 20mg / L, and continue to culture ...
Embodiment 2
[0031]The bacterial agent is composed of aerobic microorganisms, and the aerobic microorganisms are: Bacillus megaterium, Arthrobacter simplex, Aeromonas hydrophila, Alealigenes eutrophus and Pseudomonas fluorescens (Pseudomonas fluorescens), the weight ratio is 1:1:2:6:12, and the balance is the culture solution containing nitrobenzene.
[0032] The culture solution is calculated by weight percentage: glucose 0.05%, peptone 0.02%, beef extract 0.03%, NaCl 0.3%, NaCl 2 HPO 40 0.3%, KH 2 PO 4 0.1%, MgSO 4 .7H 2 O 0.02%, pH7.5.
[0033] The preparation method of bacterial agent: the bacterial agent of 5% by weight is inoculated in the Erlenmeyer flask that the sterilized culture fluid of 150ml is housed, and the initial concentration of nitrobenzene in the culture fluid is 80mg / L, and gauze pad is sealed, and is placed in 28 ℃ in a constant temperature shaking incubator for 4 days, then take out 1 / 5 of the culture solution, and then add the same amount of culture solutio...
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