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Reagent kit for detecting esophageal cancer susceptibility

A kit and esophageal cancer technology are applied in the field of kits for detecting susceptibility to esophageal cancer, and can solve problems such as affecting the susceptibility of tumor diseases, malignant transformation of cells, and damage to gene integrity.

Inactive Publication Date: 2008-02-27
XINBAXIANG SHANGHAI MOLECULAR MEDICAL TECH SHANGHAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Inherited or acquired defects in the DNA repair pathways involved in XRCC1 will destroy the integrity of genes, leading to malignant transformation of cells, thereby affecting the susceptibility of individuals to neoplastic diseases

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1. Use of detection kit

[0023] Step 1: Extraction of DNA template

[0024] The genomic DNA of oral epithelial cells was extracted by silica gel adsorption method.

[0025] Step 2: Fluorescence quantitative PCR reaction

[0026] Use a fluorescent quantitative PCR detection kit that can detect esophageal cancer susceptibility, which contains the following primer pairs and fluorescent probes:

[0027] Sense primer: 5'-TTAGAAGGTGACAGTGACCA-3' (SEQ ID NO: 2) Tm value is 56°C

[0028] Antisense primer: 5'-TCTCAACCCTACTCACTCA-3' (SEQ ID NO: 3) Tm value is 56°C

[0029] Fluorescent probe: 5'-GCTCTCTTCTTCAGCTGGATC-3' (SEQ ID NO: 4) Tm value is 64°C

[0030] The fluorescence quantitative PCR reaction system is a total volume of 10μl, containing 2μl of DNA template with a concentration of 20ng / μl, 1μl 10X fluorescence quantitative PCR reaction buffer, 0.1μl 25mM dNTP mixture, 0.6μl 25mM MgCl 2 Solution, 0.025μl (5units / μl) Taq DNA polymerase, 20μM sense primer and antisense ...

Embodiment 2

[0034] Example 2. Services to guide people to actively prevent esophageal cancer

[0035] At present, this service has been launched in Shanghai Zhujian Biological Engineering Co., Ltd.

[0036] Step 1: DNA extraction

[0037] Conduct pre-service consultations for the tested persons, who will sign an informed consent form and fill in a questionnaire about life and eating habits. Use the oral sampling swab to sample oral epithelial cells according to the instructions in the sampling box, and use the silica gel adsorption method for DNA extraction of oral epithelial cells.

[0038]Step 2: Genotyping testing

[0039] Using the kit provided by the present invention, the rs1799782 SNP site on the XRCC1 gene of the DNA of the testee is detected by fluorescent quantitative PCR to determine the genotype of the SNP site.

[0040] Step 3: Instruct people to take the initiative to prevent esophageal cancer

[0041] Through the analysis of the SNP genotype of the tested person, a genotyping t...

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PUM

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Abstract

The invention discloses a kind of reagent box for detection of susceptibility to esophageal cancer. The reagent box comprises of specific primer pairs for the detection of the 146th SNP locus in ERCC2 SEQ ID NO: 1 of XRCC1 gene, conventional components for fluorescence quantitative PCR. The reagent box by this invention can predict individual susceptibility to esophageal cancer through the detection of individual portable type of SNP locus gene in XRCC1 gene.

Description

Technical field [0001] The present invention relates to a kit for detecting disease susceptibility, in particular to a kit for detecting susceptibility to esophageal cancer, by detecting X-ray repair cross--complementing gene 1 (XRCC1) Single nucleotide polymorphism (SNP) sites are used to predict the susceptibility of individuals to esophageal cancer. Background technique [0002] Esophageal cancer is a malignant tumor that originates in the esophagus. Squamous epithelial cancer is more common. It is a common disease in some countries and regions in the world. In clinical practice, progressive dysphagia is the most typical symptom. China is a country with a high incidence of esophageal cancer in the world and one of the countries with a high mortality rate of esophageal cancer in the world, with an average annual mortality rate of 14.59 per 100,000. The epidemiology of esophageal cancer has the following characteristics: ①Regional distribution, for example, the incidence rate in...

Claims

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Application Information

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IPC IPC(8): G01N21/64C12Q1/68
Inventor 冯哲民邹祖烨
Owner XINBAXIANG SHANGHAI MOLECULAR MEDICAL TECH SHANGHAI
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