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Industrial cultivation method for Gloeostereum incarnatum S. Ito et Imai

The invention relates to a technology for the cultivation and cultivation method of Glycine, which is applied in the field of edible fungus cultivation and production, and can solve the problems of undeveloped industrial cultivation technology, and achieve the effects of stable and controllable product quality, high food safety, and shortened cultivation time.

Inactive Publication Date: 2008-01-09
CHINA NAT RES INST OF FOOD & FERMENTATION IND CO LTD
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  • Summary
  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the production of Bacteroides sarcoidium in my country is mainly wild and artificial cultivation, and the industrial cultivation technology has not yet been successfully developed.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Liquid culture:

[0031] (1) First-level seed culture: Pick a piece of the slanted strain of Bacterus colloides and insert it into a 500-milliliter shaker flask equipped with 100 milliliters of medium to carry out first-level seed culture, culture conditions: rotary shaker 100 rpm , Cultivate about 50 hours at 25°C; (2) secondary seed culture: insert the primary shake flask seeds with 10% inoculum amount into 3 liter shake flasks with 1 liter of medium for cultivation, culture conditions: rotary shake Bed 120 revs / min, 25 ℃ cultivated about 52 hours; (3) seed tank cultivation: with 10% inoculum size, it is the seed tank of 150 liters that the total volume that 100 liters of fermentation mediums are housed is inserted secondary seed. The culture conditions are: culture temperature 25°C, stirring speed 120 rpm, ventilation volume (V / V) 1:0.4, tank pressure 0.05Mpa, culture for 40 hours;

[0032] Inoculation: In the sterile space, use a sterile hose to insert the liquid b...

Embodiment 2

[0042] Liquid culture:

[0043] (1) First-level seed culture: Pick a piece of the slanted strain of Bacterus colloides and insert it into a 500-milliliter shaker flask equipped with 100 milliliters of medium to carry out first-level seed culture, culture conditions: rotary shaker 100 rpm , Cultivate about 48 hours at 24°C; (2) secondary seed cultivation: insert the primary shake flask seeds with 10% inoculum size into 3 liter shake flasks with 1 liter of medium for cultivation, culture conditions: rotary shake Bed 120 revs / min, 24 ℃ cultivated about 48 hours; (3) seed tank culture: with 10% inoculum size, it is the seed tank of 150 liters that the total volume that 100 liters of fermentation mediums are housed is inserted secondary seed. The culture conditions are: culture temperature 24°C, stirring speed 90 rpm, ventilation volume (V / V) 1:0.4, tank pressure 0.05Mpa, culture for 40 hours;

[0044] Inoculation: In the sterile space, use a sterile hose to insert the liquid bact...

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Abstract

The present invention belongs to the field of edible fungus production method, in particular, it relates to an industrial cultivation method of elm fungus. It is characterized by that said invention adopts a liquid inoculation technique and utilizes an industrial cultivation condition to implement industrial production and cultivation of elm fungus. Said method includes the following steps: adding liquid inoculum into sterilized solid culture bag, placing the solid culture bag into a culture room with 25-28deg.C, controlling air relative humidity, making culture for 8-15 days, then placing the solid culture bag into a carposporophyle culture room, controlling temperature and humidity for 5-8 days, in fungus bag primodium-formed position opening, regulating temperature to 18-23 deg, controlling humidity and making it be 94-97%, at the same time making irradiation by using scattered light for 16-20 days, then the carposporophyle can be formed.

Description

technical field [0001] The invention belongs to the field of cultivation and production of edible fungi, and in particular relates to a method for industrialized cultivation of the sarcophagus sarcoidium. Background technique [0002] Gloeostereum incarnatum, also known as elm ear and elm mill. Belongs to Basidiomycotina, Phytomycetes, Phyllomycetes, Pleurophyceae, and Glycotastella genus. Wild meat red glue bacteria are mainly distributed in Northeast China and Hokkaido, Japan, Tieling, Qingyuan, Xinbin, Benxi and Fushun in Liaoning Province, Tonghua, Hunjiang, Fusong, Changbai and Antu in Jilin Province, Heilongjiang Province The eastern mountainous area of ​​China is the main producing area of ​​yuer, and a small amount is also distributed in Xinjiang. [0003] Bacillus bacilli is a famous fungus with both medicinal and edible functions. Ulmus has a long history of being introduced into traditional Chinese medicine. In the "Compendium of Materia Medica" written by Li ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04C12N1/14
Inventor 蔡永峰岳国海李绩张贵林
Owner CHINA NAT RES INST OF FOOD & FERMENTATION IND CO LTD
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