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Alpha-glycosidase inhibitor, its extraction and use

A technology of glycosidase inhibitors and extraction methods, which is applied in the field of biomedicine to achieve the effect of rich sources of raw materials and simple extraction methods

Inactive Publication Date: 2011-02-09
广元天湟山核桃食品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] 2. Non-insulin drugs: Although a lot of progress has been made in the research of new dosage forms and new routes of administration of insulin, it is still difficult to meet the needs of many types of diabetic patients with insulin alone
Preliminary data retrieval shows that there is no report on the use of walnuts (including soaked walnuts and iron walnuts) in the field of medicine to inhibit the activity of α-amylase and α-glucosidase at home and abroad.

Method used

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  • Alpha-glycosidase inhibitor, its extraction and use
  • Alpha-glycosidase inhibitor, its extraction and use
  • Alpha-glycosidase inhibitor, its extraction and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] 1. Extraction method: Soak walnuts and iron walnuts, shell them and take kernels, bake overnight at 45°C, weigh 100g each, add 300ml of 95% ethanol, extract on a shaking table at 45°C for 5 hours, filter, concentrate, and dry at 45°C. Get 2.36g of soaked walnut kernel crude extract and 2.07g of iron walnut kernel crude extract. This crude extract was used for activity assays.

[0020] 2. Activity determination:

[0021] (1) Inhibition of α-amylase activity

[0022] ① Solution configuration:

[0023] Starch solution: soluble starch was dissolved in 0.2M citric acid-disodium hydrogen phosphate buffer solution, pH=6.4, heated in a boiling water bath for 5 minutes, cooled to room temperature, and distilled water was added to a final concentration of 1% (W / V).

[0024] Iodine solution: 0.5g I2 and 5.0g KI are dissolved in 50ml distilled water, stored in a brown bottle, diluted with distilled water at a ratio of 1:200 before use.

[0025] α-amylase: Beijing Aoboxing Biote...

Embodiment 2

[0056] 1. Extraction method: Dried iron walnut oil residue, powdered, sieved, weighed 100g, added 300ml of 95% ethanol, leached on a shaker at 45°C for 5 hours, filtered, concentrated, dried at 45°C to obtain a crude extract Material 5.01g, this crude extract was used for activity determination.

[0057] 2. Activity determination:

[0058] ① solution configuration: with embodiment one.

[0059] ② Activity assay method: the same as in Example 1.

[0060] ③Experimental results:

[0061] (1) Inhibition of α-amylase activity

[0062] (2) Inhibit the activity of α-glucosidase

[0063] Note: OD 标 =0.235

Embodiment 3

[0065] 1. Extraction method: Dry the iron walnut shell, powder it, sieve it, weigh 28g, add 84ml of 95% ethanol, extract on a shaking table at 45°C for 5 hours, filter, concentrate, and dry at 45°C to obtain 0.23g of extract , this extract was used for activity determination.

[0066] 2. Activity determination:

[0067] ① solution configuration: with embodiment one.

[0068] ② Activity assay method: the same as in Example 1.

[0069] ③Experimental results:

[0070] (1) Inhibition of α-amylase activity

[0071] (2) Inhibit the activity of α-glucosidase

[0072] Note: OD 标 =0.235

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Abstract

The invention is concerned with the method of preparation of the alpha-glycosidase inhibitor extracts from walnut. It contents the active ingradient of the shell and kernel of the walnut (also includes Juglans regia L., Juglans cathayensis Dode, and Juglans mandshurica Maxim). Thealpha-glycosidase inhibitor is extracted from the draff after oil fired by water, alcohol, or water-alcohol solution, through filtration, concentration, and drying at last. It can use for prepareing health products for diabetes therapy or blood suger controlling.

Description

technical field [0001] The invention belongs to the field of biomedicine, and relates to an alpha-glucosidase inhibitor extracted from walnuts, an extraction method and application thereof. Background technique [0002] Diabetes has become a serious public health problem worldwide. There are about 132 million people suffering from diabetes worldwide, and it will reach 240 million in 2010. In my country, according to the latest epidemiological survey, the incidence of diabetes in my country has risen from 0.67% 15 years ago to 3.21% in recent years, of which type II diabetes patients account for more than 95% of the total, and there is no significant difference between men and women . According to statistics, the number of diabetics in my country was 6.5 million in 1978, about 7.5 million in the mid-1980s, and reached 15 million in 1995. In recent years, the number of diabetics has been increasing by more than 1 million every year. By the end o...

Claims

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Application Information

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IPC IPC(8): A61P3/10A61K38/02
Inventor 何开泽谭健付田
Owner 广元天湟山核桃食品有限公司
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