Section staining method for striped skeletal muscles

A technique for section dyeing and skeletal muscle, applied in the field of section dyeing of skeletal muscle striated, can solve the problems of low quality, increased workload, fine striated, etc., and achieve the effect of high success rate

Inactive Publication Date: 2007-09-26
孝感学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the workflow of HE staining is very cumbersome (Bing Lujun, Gao Yingmao, Guo Yuqi, Zhang Xiaoli. Techniques for Improving the Quality of Paraffin Sections in Histology Experiment Teaching [J]. Laboratory Exploration, 2004, 23(12): 152-153. ), the longitudinal section can show the horizontal stripes of skeletal muscle, but the horizontal stripes are relatively small and the quality is not high
For mass production, it is necessary to select from them, which increases the workload.

Method used

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  • Section staining method for striped skeletal muscles
  • Section staining method for striped skeletal muscles
  • Section staining method for striped skeletal muscles

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1: Take the bullfrog skeletal muscle and put it into the fixative solution. After fixing it for 4 hours, take out the skeletal muscle and cut it into 5 mm tissue pieces, then put it into the fixative solution and fix it for 20 hours. After rinsing with running water, put it in potassium permanganate hematoxylin staining solution for 12 days, then use 70% alcohol for 30 minutes, wash with running water for 24 hours, step by step from 70% alcohol, 80% alcohol, 90% alcohol to each liquid It lasted 2 hours, and then stained with eosin solution for 3 days. Skeletal muscle tissue pieces were dehydrated in 95% alcohol and 100% alcohol for 2 hours each, soaked in xylene for 16 hours, then soaked in soft wax twice, each time for about 60 minutes, a total of 2 hours; hard wax soaked twice, each time About 60 minutes, a total of 2 hours. Routine paraffin embedding. Cut the wax block into 5 μm thick wax ribbons with a microtome. Cut the wax tape into 0.8-1 cm long wax sh...

Embodiment 2

[0034] Embodiment 2: Take the bullfrog skeletal muscle and put it into the fixative solution. After fixing for 6 hours, take out the skeletal muscle and cut the tissue into 3mm blocks, and then put it into the fixative solution for 6 hours. After rinsing with running water, put it in potassium permanganate hematoxylin staining solution for 10 days, then use 70% alcohol for color separation for 15 minutes, wash with running water for 20 hours, and gradually change from 70% alcohol, 80% alcohol, 90% alcohol to each solution It lasted 2 hours, and then stained with eosin solution for 2 days. The skeletal muscle tissue pieces were dehydrated in 95% alcohol and 100% alcohol for 2 hours each, soaked in xylene for 14 hours and then soaked in soft wax twice, 45 minutes each time; hard wax twice, 45 minutes each time. Routine paraffin embedding. Cut the wax block into 6 µm thick wax ribbons with a microtome. Cut the wax tape into 0.8-1 cm long wax slices and paste them on the glass s...

Embodiment 3

[0035] Embodiment 3: Take the bullfrog skeletal muscle and put it into the fixative solution. After fixing it for 5 hours, take out the skeletal muscle and cut it into 4 mm tissue pieces, then put it into the fixative solution and fix it for 15 hours. After rinsing with running water, put it in potassium permanganate hematoxylin staining solution for 10 days, then use 70% alcohol to separate the color for 20 minutes, rinse with running water for 25 hours, step by step from 70% alcohol, 80% alcohol, and 90% alcohol to each solution. It lasted 2 hours, and then stained with eosin solution for 3 days. The skeletal muscle tissue blocks were dehydrated in 95% alcohol and 100% alcohol for 2 hours each, soaked in xylene for 15 hours and then soaked in soft wax twice, 40 minutes each time; hard wax twice, 60 minutes each time. Routine paraffin embedding. Cut the wax block into 4 µm thick wax ribbons with a microtome. Cut the wax tape into 0.8-1 cm long wax slices and paste them on t...

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Abstract

This invention relates to one skeleton muscle slice dyeing method, which comprises the following steps: taking skeleton muscles into fix liquid for four to six hours and then cutting the muscles into organism blocks for three to five millimeter and then putting into fix liquid for eight to eighteen hours; taking skeleton muscles organism block flow by clearing and into potassium permanganate dyeing liquid for ten to twelve days; then using alcohol liquid with seventy percent for 15 to 30 minutes for clearing orderly through alcohol liquid with 70, 80 and 90 percent into eosin dyeing liquid; then putting the skeleton muscles organism into alcohol for 95 percent and 100 percent alcohol for two hours and dipping the organism block into dimethylbenzene and then into soft wax twice and hard wax for twice.

Description

technical field [0001] The invention relates to a method for staining slices of skeletal muscle stripes, belonging to the technical field of biological slices. Background technique [0002] Skeletal muscle tissue section plays a pivotal role in the teaching process of histology, pathology, biology, etc. It is a very complicated and cumbersome technique with a large demand in the teaching process, and the technical requirements are very strict. [0003] The staining method during the preparation process is a critical step in the visualization of striations in skeletal muscle. The staining methods currently used for skeletal muscle mainly include three types of staining methods under light microscope, fluorescence microscope and electron microscope observation. The staining methods used for observation under the fluorescence microscope and electron microscope can clearly show the morphology of tissue cells according to their own functions, and there are many staining methods,...

Claims

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Application Information

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IPC IPC(8): G01N1/30G01N33/48
Inventor 刘红云张静张明星
Owner 孝感学院
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