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Preparing method of heterotransplantation of bones

A technology of bone transplantation and xenografts, which is applied in the field of preparation of xenograft bone graft materials, can solve the problems of no mention of xenograft antigen treatment methods, no removal of bone material fat, and inability to completely remove xenogeneic bone antigens and fat, etc., to achieve weight loss Small effect, effect of reducing immune rejection of transplantation

Inactive Publication Date: 2007-09-12
WEST CHINA HOSPITAL SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The problems with this method are: 1. There is no mention of the treatment of xenograft antigens other than α-Gal; 2. The purpose of washing with water and alcohol is only to remove synovial fluid and soluble lipid contamination, not to remove bone material fat in
Ther

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  • Preparing method of heterotransplantation of bones
  • Preparing method of heterotransplantation of bones
  • Preparing method of heterotransplantation of bones

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1: Detection of the expression and distribution of α-Gal, MHC-I and MHC-II antigens in the original pig bone

[0034] (1) Main reagents

[0035] M86: Anti-α-Gal monoclonal antibody, produced by Alexis;

[0036] SLA-1: mouse anti-pig MHC-I monoclonal antibody, produced by Serotec;

[0037] SLA-DR: mouse anti-pig MHC-II monoclonal antibody, produced by BD Company.

[0038] (2) Main materials and equipment

[0039] The specimens were obtained from the cancellous bone and ribs of the femoral condyle and ribs of fresh adult inbred Neijiang pigs aged 6 to 8 months. Neijiang pigs were purchased from the West China Basic Medical Animal Experiment Center of Sichuan University. LEICA2500E slicer, 1600 trimmer, mounter, produced by Germany LEICA.

[0040] (3) Immunohistochemical detection of α-Gal, MHC-I and MHC-II antigens

[0041] ① Specimens were fixed with 4% paraformaldehyde for 24 hours;

[0042] ② After decalcification of 0.5M EDTA at 4°C for 2-3 months, seria...

Embodiment 2

[0054] Embodiment 2: the preparation of pig bone graft material

[0055] In this example, ribs of fresh adult inbred Neijiang pigs aged 6 to 8 months were used as raw materials. Neijiang pigs were purchased from the West China Basic Medical Animal Experiment Center of Sichuan University. The specific preparation method is as follows:

[0056] (1) Remove bone marrow and blood cells

[0057] Remove the tissue and periosteum around the pig ribs under aseptic conditions, take the middle part of the ribs and keep one side of the cortex, and make a 1.5cm×0.5cm×0.4cm bone strip. Rinse with high-pressure pulsed distilled water with a pressure of 350Kpa under aseptic conditions, and rinse for about 60 minutes to remove the bone marrow and blood cells in the pork ribs and their medullary cavities;

[0058] (2) ether degreasing and washing

[0059] Under normal temperature and pressure, the pig ribs from which bone marrow and blood cells have been removed in step (1) are placed in eth...

Embodiment 3

[0064] Embodiment 3: the preparation of pig bone graft material

[0065] In this example, femoral condyles of fresh adult inbred Neijiang pigs aged 6 to 8 months were used as raw materials. Neijiang pigs were purchased from the West China Basic Medical Animal Experiment Center of Sichuan University. The specific preparation method is as follows:

[0066] (1) Remove bone marrow and blood cells

[0067] The tissue and periosteum around the porcine femoral condyle were removed under aseptic conditions, and the cancellous bone in the middle of the femoral condyle was taken to make a 0.5 cm × 0.5 cm × 0.5 cm bone block. ℃) and under sterile conditions, rinse with high-pressure pulsed saline with a pressure of 600KPa, and rinse for about 30 minutes to remove the bone marrow and blood cells in the porcine cancellous bone and its medullary cavity;

[0068] (2) ether degreasing and washing

[0069] Under normal temperature and pressure, the porcine cancellous bone from which bone ma...

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Abstract

The preparation process of heterogeneous bone transplanting material includes the following steps: 1. washing animal bone as the material with high pressure pulse distilled water or normal saline at normal pressure and room temperature and in aseptic condition to eliminate bone marrow and blood cell; 2. defatting with 95-100 wt% concentration ether solution and rinsing with aseptic water to eliminate ether; 3. ultralow temperature freezing in liquid nitrogen at normal pressure; and 4. irradiating with gamma ray.

Description

technical field [0001] The invention belongs to the field of bone graft materials, in particular to a preparation method of heterogeneous bone graft materials. Background technique [0002] Bone defects caused by trauma, tumors and artificial joint revisions have always been one of the most difficult problems for orthopedic surgeons. Although there are many methods for the reconstruction of bone defects, bone transplantation is considered to be one of the most effective methods. Autologous bone grafting has been used as a standard method of bone grafting due to its good osteogenic activity, osteoinductivity, and osteoconductivity. However, due to the limited source of autologous bone and possible complications such as local bleeding and nerve injury, the clinical application of autologous bone is limited. Allogeneic bone transplantation is another commonly used bone graft method in clinical practice. However, allogeneic bone transplantation not...

Claims

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Application Information

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IPC IPC(8): A61L27/36
Inventor 裴福兴程惊秋冯卫廉永云周宗科
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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