Method for preparing concatenated active bifidobacterium product
The technology of bifidobacteria and bifidobacteria breve is applied in the field of preparation of multiple active bifidobacteria, which can solve the problems of reducing the content of bacteria and affecting physical health, and achieves strong gastric acid resistance and the number of viable bifidobacteria. The effect of long storage period at high and normal temperature
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[0019] Prepare 1000g of multi-linked active bifidobacteria product with a number of viable bacteria of 1.0×10 8 cfu / g, and it is composed of Bifidobacterium breve, Bifidobacterium infantis, Bifidobacterium longum and Lactobacillus tetralogy, and the ratio of bacteria is basically 1:1:1:1.
[0020] Set the number of live Bifidobacterium breve dry powder to 2.3×10 9 cfu / g, the number of live Bifidobacterium infantis dry powder is 1.3×10 9 cfu / g, the number of live Bifidobacterium longum dry powder is 2.03×10 12 cfu / g, the number of live bacteria in dry powder of Lactobacillus is 1.63×10 8 cfu / g.
[0021] According to the product formula, weighed separately after sterilization in an environmental condition with a cleanliness of class 100:
[0022] Bifidobacterium breve dry powder: 1.0×10 8 ÷4÷(2.3×10 9 )×1000=10.9g
[0023] Dry powder of Bifidobacterium infantis: 1.0×10 8 ÷4÷(1.3×10 9 )×1000=19.2g
[0024] Bifidobacterium longum dry powder: 1.0×10 8 ÷4÷(2.03×10 12 )×1000=0.02g
[002...
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