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Adrenergic receptor SNP for improved milking characteristics

A technology of adrenaline and receptors, which is applied in the determination/testing of microorganisms, organic chemistry, fermentation, etc., and can solve problems such as the inability to predict the characteristics of lactation

Inactive Publication Date: 2008-10-01
代表亚利桑那州立大学的亚利桑那州立大学董事会 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, while it is possible to measure overall statistical averages of the lactation characteristics of female calves of a particular breed, it has not been possible to predict with any degree of success the lactation characteristics of a particular offspring produced by breeding a cow with a particular bull

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1. Evaluation of mRNA levels in white blood cells of cows with fast and slow lactation rates.

[0028] Eight animals were selected according to the duration of their lactation and divided into fast and slow groups. For initial assays, slow lactating animals were numbered 90, 264, 279 and 321. Fast lactating animals were numbered 272, 273, 281 and 289. Their blood (approximately 15 mL) was collected in EDTA-filled Vacutainers (BD, Franklin Lakes, NJ). The known sequences of NCBI and the "Oligo" program were used to design primer sets specific for GAPDH, α2-, β1-, β2-adrenergic receptors and β-arrestin.

[0029] RNA was isolated from blood samples using the RNeasy RNA isolation method (Qiagen, Valencia, CA). Mix 1 volume of whole blood with 5 volumes of erythrocyte lysis buffer. This mixture was incubated on ice and mixed by brief vortexing twice during incubation. The mixture was then centrifuged at 400 g for 10 minutes at 4°C. Discard the supernatant and s...

Embodiment 2

[0035] Example 2. Relationship between A11C genotype and dairy cow SCS and / or lactation speed

[0036] Bovine DNA was obtained from the Cooperative Dairy DNA Repository (CDDR) group (GeneEvaluation and Mapping Laboratory, Bldg. 200Rm 2A, ARS-USDA, BARC-EastBeltsville, MD 20705). SCS phenotypes were obtained for all CDDR animals. Lactation velocity (MS) data are also available for a subset of CDDR animals. CDDR animals with MS data were first genotyped by A11C assay, while the remaining CDDR animals were divided into subgroups of "high" and "low" somatic cell score (SCS) phenotype categories. DNA samples were obtained and assayed. Data sets were assigned and analyzed in duplicate. Analysis revealed a relationship between A11C and SCS.

[0037] 663 animals from CDDR were genotyped at the A11C locus with one of the following primer pairs:

[0038] TGGAACTGGCTGAACTGACA (SEQ ID NO 1)

[0039] AGTTGATGGCTTCCTTGTGG (SEQ ID NO 2)

[0040] AGGTCCGCTCGCTGAGG (SEQ ID NO 3)

[004...

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PUM

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Abstract

Disclosed herein is a method for screening for the allele associated with a desired SCS phenotype, which comprises: obtaining a DNA sample from a bull to be tested for the desired SCS phenotype; and detecting the presence of an adenine at position 11 in a gene encoding a bovine beta2-adrenoreceptor. Also disclosed is a milking attribute PCR-RFLP kit containing a pair of primers which flank the 11 nucleotide position of the bovine beta2-adrenoreceptor gene, and a restriction enzyme specific for the CCCGGG site, which can be SmaI.

Description

[0001] Cross References to Related Applications [0002] none. [0003] Statement Regarding Federally Funded Research or Development [0004] none. Background of the invention [0005] The present invention relates generally to the fields of molecular biology, human and bovine genetics, and desirable lactation characteristics. Specifically, the present invention provides methods for predicting lactation characteristics of cows using genetic methods. [0006] This application refers to a number of publications or patents to describe the state of the art to which this invention pertains. Each publication or patent is incorporated herein by reference. Full citations to scientific publications are listed in the text or at the end of the specification. [0007] Men bred domestic animals to improve the useful qualities of these animals. One very useful quality for cows is their lactation characteristics, which include milk volume and lactation rate. Many cows are artificially...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H21/02C07H21/04C12P19/34C12Q1/68
CPCC12Q1/6888C12Q1/683C12Q2600/124C12Q2600/156
Inventor R·J·科利尔M·洛希斯M·格罗斯
Owner 代表亚利桑那州立大学的亚利桑那州立大学董事会
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