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Preparation process of cow mammitis vaccine

A technique for bovine mastitis and vaccine, which is applied in the field of preparation of bovine mastitis vaccine, can solve the problems such as not fully exerting the advantages of core universal antigen Escherichia coli, and achieve the effects of preventing bovine mastitis, reducing endotoxin and reducing side effects

Inactive Publication Date: 2008-05-14
HENGXIN GLOBAL AGRI SCI & TECH
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AI Technical Summary

Problems solved by technology

But because J5 Escherichia coli (E.Coli) vaccine preparation adopts traditional vaccine preparation method, still has all some drawbacks of traditional method, does not give full play to the advantage of core common antigen Escherichia coli (E.Coli) vaccine

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 1. Extract the plasmid containing the sucrose-6 fructose transferase (sacB) gene

[0022] Purchase a strain of Escherichia coli (E.coli) DH5F' (ATCC#77281), which contains the plasmid pHSS21, which has a hygromycin resistance marker and a sucrose-6 fructose transferase (sacB) gene, and thus is sensitive to sucrose sex. See (Schweizer, H.P. (1992) Pseudomonas cyanides allelic exchange using Coli1-type vectors and cassettes with portable oriT and sacB markers. Molecular Microbiology 6(9) 1195-1204). Using conventional molecular biology methods, the plasmid pHSS21 was extracted and used as the plasmid used in the next step of transformation.

[0023] 2. Preparation of Escherichia coli (E.coli) J5 competent cells

[0024] (a) Inoculate Escherichia coli (E.coli) J5 onto 40 ml of TSA medium containing kanamycin, and cultivate overnight at 37° C.;

[0025] (b) The next day, inoculate overnight Escherichia coli (E.coli) J5 culture in a flask of 40ml LB medium, cultivate over...

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Abstract

In the preparation process of cow mastitis vaccine, Gram-negative bacterium containing core universal antigen is utilized and transformed with genetic plasmid containing sucrose-6 fructose transferase. Under the induction of sucrose, Gram-negative bacterium suicides and the Gram-negative bacterium with core universal antigen is collected to prepare the cow mastitis vaccine. The said process produces no damage to immunogenicity of the vaccine and no substituted or non-natural vaccine antigen. Using non-toxic sucrose as chemical inducing matter can reduce the endotoxin in and side effect of the vaccine. The vaccine stimulates the immunological system to resist coibacillus, salmonella and other Gram-negative bacteria causing cow mastitis.

Description

technical field [0001] The invention relates to a preparation method of a vaccine, in particular to a preparation method of a bovine mastitis vaccine. Background technique [0002] Bovine mastitis is a serious and costly disease on dairy farms around the world. This disease usually has the possibility of onset at various stages of lactation, and 50% of bovine mastitis occurs in the early stage of lactation. At all stages of lactation, mammary gland secretions are a good medium for bacterial growth. When external bacteria successfully pass through the nipple and ducts and enter the mammary gland tissue, it will cause infection and inflammation in the mammary gland. [0003] Cases of clinical bovine mastitis are caused by bacteria in the environment, and the overall percentage of bacterial mastitis incidence has risen sharply in recent years. Bacteria are microorganisms that exist in the environment. However, after the cow gives milk, since the pores of the mammary gland ar...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/02A61K39/108A61P31/04
Inventor 詹古拉
Owner HENGXIN GLOBAL AGRI SCI & TECH
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