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Halymenia callus growth method

A callus and sea membrane technology, applied in the field of large seaweed sea membrane breeding, can solve the problems of increased disinfectants and antibiotics, death, lack of growth and differentiation, etc., to achieve the effect of easy application and high technical content

Inactive Publication Date: 2007-11-14
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with higher plants, the relative difficulties of seaweed tissue culture are: 1. It is difficult to obtain sterile materials: the surface of seaweed has many parasites and grows firmly, so it must be scrubbed vigorously, and the outer cells of seaweed are not protected by cuticles. At the same time, due to the large number and variety of parasitic microorganisms, the amount of disinfectants and antibiotics used needs to be increased, which can easily cause deformity or death of explants; 2. The basic research of seaweed is backward and lacks control knowledge of growth and differentiation
Due to the technical difficulty in the control of callus subculture conditions, although callus can be obtained by induction, the callus generally cannot continue to grow after trying to separate the callus from the explant

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Collect sea membrane fronds. After rinsing with sterile seawater for 5 times, cut into small pieces of 3 mm in size with a blade, place them in a petri dish filled with 20 ml of PES culture solution (Provasoli's enriched seawater), at 20°C, with a photoperiod of 12:12h =L:D, light intensity 50μmol photons (photons) m -2 the s -1 under cultivation. After two to three weeks, filamentous callus grows from the edge of the cut. This callus was cut with a blade under a microscope, and placed in a 150 ml Erlenmeyer flask to carry out suspension culture of this callus. The temperature, culture medium, light intensity and photoperiod of cultivation were the same as the above-mentioned conditions for inducing callus. After the callus grows to a size of 5 mm in diameter, it is smashed, the culture medium is replaced every two weeks, and it is stored indoors. The temperature, culture medium, light intensity and photoperiod of the culture are the same as the above-mentioned condi...

Embodiment 2

[0021] Collect sea membrane fronds. After rinsing with sterile seawater for 5 times, cut into small pieces with a size of 3 mm, place them in a petri dish filled with 20 ml of PES culture solution (Provasoli's enriched seawater), at 25°C, with a photoperiod of 12:12h =L:D, light intensity 20μmolphotons·m -2 the s -1 under cultivation. After two to three weeks, filamentous callus grows from the edge of the cut. This callus was cut with a blade under a microscope, and placed in a 150 ml Erlenmeyer flask to carry out suspension culture of this callus. The temperature, culture medium, light intensity and photoperiod of cultivation were the same as the above-mentioned conditions for inducing callus. After the callus grows to a size of 2 mm in diameter, it is smashed, the culture medium is replaced once a week, and it is stored indoors. The temperature, culture medium, light intensity and photoperiod of the culture are the same as the above-mentioned conditions for inducing callu...

Embodiment 3

[0023] Collect sea membrane fronds. After rinsing with sterile seawater for 5 times, cut into small pieces of 3 mm in size with a blade, place them in a petri dish filled with 20 ml of PES culture solution (Provasoli's enriched seawater), at 15°C, with a photoperiod of 12:12h =L:D, light intensity 80μmol photons·m -2 the s -1 under cultivation. After two to three weeks, filamentous callus grows from the edge of the cut. This callus was cut with a blade under a microscope, and placed in a 150 ml Erlenmeyer flask to carry out suspension culture of this callus. The temperature, culture medium, light intensity and photoperiod of cultivation were the same as the above-mentioned conditions for inducing callus. After the callus grows to a size of 10 mm in diameter, it is crushed, the culture medium is replaced once a week, and it is stored indoors. The temperature, culture medium, light intensity and photoperiod of the culture are the same as the above-mentioned conditions for ind...

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Abstract

The Halymenia seedling culturing process with Halymenia callus includes inducing in specific conditions to produce filamentous callus; separating the callus and controlling lighting, temperature, nutritive salt and other conditions to realize the secondary culture and maintenance of callus; suspending culture of the callus for fast amplification; spraying the suspension cultured callus to attaching medium for quiet water culture and subsequent flowing water culture after attachment; and altering the culture conditions to induce regeneration to form new seedling. The said Halymenia seedling culturing process is effective, has no character segregation and is favorable to fine variety breeding.

Description

technical field [0001] The invention relates to a seedling raising technique of large seaweed sea film, in particular to a method for cultivating sea film seedlings by using callus. Background technique [0002] Sea film is a type of edible seaweed and can be used as a raw material for extracting alginate. At present, the acquisition of algal bodies is mainly to collect natural resources, and artificial breeding has not yet been carried out. The main reason is that the spore yield of sea film is relatively low, and the amount of artificially cultivated sporelings is small, which cannot form a culture scale. For its seedling cultivation, tissue culture techniques similar to those of higher plant tissues can be used. Although the tissue culture of macroalgae started in the 1950s, the progress of tissue culture research is relatively slow because seaweed has many characteristics different from higher plants. Compared with higher plants, the relative difficulties of seaweed t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G33/00
Inventor 胡晓燕殷明焱夏娃
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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