Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Boea clarkeane drought-resistant and salt-tolerance related gene and its coding protein and use

A technology of Capsulatum, coding protein, applied in application, genetic engineering, plant genetic improvement, etc., can solve problems such as lack of stress-resistant resource genes, and achieve the effect of improving drought tolerance and high practical application value

Inactive Publication Date: 2007-10-24
INST OF BOTANY CHINESE ACAD OF SCI
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In my country, although the use of stress-resistant genes to cultivate stress-resistant strains of crops, forest grasses, etc. has become the main idea of ​​plant breeding, there is still a shortage of stress-resistant resource genes with independent intellectual property rights in my country.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Boea clarkeane drought-resistant and salt-tolerance related gene and its coding protein and use
  • Boea clarkeane drought-resistant and salt-tolerance related gene and its coding protein and use
  • Boea clarkeane drought-resistant and salt-tolerance related gene and its coding protein and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1, Acquisition of Drought Resistance and Salt Tolerance Related Gene BhLea1 of Capsulatum

[0031] Total RNA was extracted from the leaves of Capsulatum spp. leaves subjected to drought stress for 8 hours, and a cDNA library was constructed using the ZAP-cDNA(R) library construction kit (Stratagene, La Jolla, CA). 4800 genes were randomly selected from them, and automatically sampled on Hybond-N with BioGrid robot (BioRobotics Ltd, Cambridge, UK). +cDNA microarrays (cDNA chips) were fabricated on nylon membranes (Amersham Biosciences, Freiburg, Germany). The polyA-RNA prepared by combining the chip with the polyA-RNA of the leaves of Capsula oleracea that grew normally without drought treatment and the leaves of Capsula capsulae leaves that were drought-stressed for 8 hours 33 P-labeled probes were hybridized to analyze their dynamic changes in gene expression under normal conditions and after drought induction. The genes after drought induction were sequenced...

Embodiment 2

[0032] Example 2, BhLea1 expression pattern analysis experiment

[0033] Extract the total RNA of the leaves of Capsulatum spp. without stress treatment, drought stress for 2, 8, 24, and 72 hours, and 0.2M NaCl stress and flooding stress for 9 hours, and measure the concentration. Take equal amounts of each sample for 1% agarose gel electrophoresis, after the bromophenol blue-stained sample migrates to the 2 / 3 of the gel away from the sample hole, transfer the RNA to the nylon membrane, bake the membrane at 80°C for 120 minutes, and then transfer the RNA to the nylon membrane. The nylon membrane of the above RNA was put into a hybridization tube, and after pre-hybridization at 65°C for 2.5 h, the purified and denatured fluorescein-labeled BhLea1 probe (from 1 to 588 at the 5' end of SEQID No. 1 in the sequence table) was added. Base position nucleotide sequence), hybridize at 65°C for 24 hours, pour out the hybridization solution, wash the membrane with 2×SSC, 0.1% SDS, 0.1×SS...

Embodiment 3

[0034] Example 3 Cytological localization of BhLea1

[0035] Using PSORT computer program analysis software (Nakai and Kanehisa, Expert system for predicting protein localization sites in gram-negative bacteria. Proteins. 11 (2): 95-110, 1991) to carry out cytological localization of the protein BhLea1 encoded by BhLea1, the results It indicated that BhLea1 was localized in the cytoplasm of the leaf cells of Capsula oleifera.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses the drought defying and salt proof relative gene of Boea hygrometrica and the coding albumen. The gene one of the following nucleotide sequence: 1) SEQ ID NO: 1 of the DNA sequence in sequence table; 2) SEQ ID NO:2 of the amino acid sequence in sequence table; 3)nucleotide sequence intercrossing with the DNA sequence. The BhLeal could regulate the drought defying and salt proof ability of plants.

Description

technical field [0001] The present invention relates to a plant gene and its coded protein and its application, in particular to a drought-resistant and salt-tolerant related gene and its coded protein derived from Capsulatum spp. Background technique [0002] Under the conditions of drought and salt stress, in addition to causing changes in metabolism, plants can also activate the expression of some other genes in the body, causing new protein accumulation in the vegetative tissues of plants. The accumulation of these proteins can improve the adaptability of plants to adverse environments, such as LEA protein, which is a coded product of a drought-salt-inducible gene (Yu Jianing, Shan Lun. LEA protein and plant drought resistance bioengineering progress 2002, 22( 2): 10-14). LEA protein has a high degree of hydrophilicity, and can capture a large amount of water molecules into the cells under the stress of drought and salt stress, protect the biological macromolecules in t...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C07H21/04C07K14/415C12N15/63A01P21/00
Inventor 邓馨马克平王丽丽王智刘霞
Owner INST OF BOTANY CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com