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Affinity based system for detecting particulates in a fluid

a technology of affinity binding and analytical system, applied in the field of detecting, characterizing and quantifying particulates suspended in fluids, can solve the problems of compromising the safety and reliability of available drugs, negatively affecting the performance and useful life of complex machinery,

Inactive Publication Date: 2006-08-31
TECAN TRADING AG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The invention provides an integrated, analytical apparatus for detecting particulate matter, particularly cells, in fluids. The apparatus includes a platform with a surface that has a detection chamber, where a specific binding reagent is deposited to bind to the cells. The platform can be a rotatable disk. The apparatus also includes fluid sample input means, overflow reservoirs, wash buffer reservoirs, fluid waste receptacles, or reservoirs containing detectable labeling reagents or moieties for detecting cells. The apparatus can detect, quantitate, and characterize the effects of test compounds on cells.

Problems solved by technology

Dust and dirt particles in water, oil or other industrial fluids can negatively impact on the performance and useful lifetime of complex machinery.
Pyrogens, including bacterial cells, in pharmaceutical products, or manufacturing facilities making such products, can compromise the safety and reliability of available drugs.

Method used

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  • Affinity based system for detecting particulates in a fluid
  • Affinity based system for detecting particulates in a fluid
  • Affinity based system for detecting particulates in a fluid

Examples

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Effect test

example 1

Cell Counting, Identification and Monitoring

[0150] Apparatus and methods for identifying particular cells or cell types in a biological sample are provided by the invention. A platform as described herein and in co-owned and co-pending U.S. Ser. No. 08 / 768,990, filed Dec. 18, 1996, incorporated by reference, is prepared by having a surface advantageously comprising a detection chamber adsorbably coated with monoclonal antibody specific to E. coli, the remaining sites on the surface comprising the detection chamber being blocked with bovine serum albumin (BSA). A milk sample is introduced onto the platform via a sample inlet port and brought into contact with the detection chamber comprising the surface coated with the antibody. The milk is incubated in this chamber for 30 min. The platform is then rotated to remove unwanted materials from the detection chamber and into a fluid waste receptacle with which the detection chamber is in fluid communication. An amount of a buffer appropr...

example 2

Somatic Cell Counting

[0151] An example of an assay for a mammalian cell in a biological fluid is the detection of somatic cell in a sample of cow's milk. The assay system is illustrated in FIGS. 2 and 2A and consists of a sample entry port 21, wash buffer chambers 27 and 29 (containing a wash buffer solution of 25 mM potassium hydrogen (KH) phthalate, pH 5 / 0.001% Triton X-100), a dye chamber 28 (containing a staining solution of 0.002% ethidium bromide in KH-phthalate buffer), and a binding / detection chamber 24, which incorporates a binding surface which has been modified with a specific binding reagent comprising antibodies specific for bovine leukocyte cell surface antigens. The platform also comprises an overflow reservoir 23 and waste receptacle 25. The milk sample is introduced, optionally after pretreatment, for example, to remove fat globules or other non-specific particulates, into the sample entry port on the platform. Platforms of the invention can be provided with wash b...

example 3

Drug Discovery

[0153] Another example of the apparatus and methods of the invention is automated evaluation of the effect of test molecules on a population of cells. Advantageously, the cells are bacterial or mammalian cells, and originate from either primary cultures (i.e. freshly harvested, particularly hematopoietic cells) or established cell lines. Viability, metabolic activation (as indicated by changes in membrane potential, intracellular pH and / or intracellular free calcium concentration) and other cellular responses are assessed using the detection means of the invention described here. Test molecules include drugs such as antibiotics, compounds being assessed for cellular toxicity, or molecules being screened to activate or block a particular receptor on a cell. The platforms or disks of the invention are used generally according to the invention as follows. Cells are cultured or isolated, stained with a suitable dye, exposed to the test molecule, and the cells then analyze...

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Abstract

This invention provides methods and apparatus for detecting and quantifying particulate matter suspended in a fluid. Specifically, the invention provides an integrated, affinity-binding based, analytical system comprising a platform for performing an affinity-binding based assay for specifically binding particulates including microbial cells, and a detection means for detecting the particulates specifically bound to a defined surface or chamber comprising the platform. Methods for using the analytical systems of the invention are also provided.

Description

[0001] This application is a divisional of U.S. Ser. No. 10 / 726,337, filed Dec. 3, 2003, now U.S. Pat. No. 6,953,550, issued Oct. 11, 2005, which is a divisional of U.S. Ser. No. 09 / 989,259, filed Nov. 20, 2001, now U.S. Pat. No. 6,656,430, issued Dec. 2, 2003, which is a divisional of U.S. Ser. No. 09 / 614,834, filed Jul. 12, 2000, now U.S. Pat. No. 6,319,468, issued Nov. 20, 2001, which is a divisional application of U.S. Ser. No. 08 / 995,056, filed Dec. 19, 1997, now U.S. Pat. No. 6,143,247, issued Nov. 7, 2000, which claims priority to U.S. Provisional Application Ser. No. 60 / 034,327, filed Dec. 20, 1996.BACKGROUND OF THE INVENTION [0002] 1. Field of the Invention [0003] This invention relates to methods and apparatus for detecting, characterizing and quantifying particulate matter suspended in a fluid. More specifically, the invention provides an integrated, affinity-binding based analytical system for detecting particulates, particularly cells, suspended in a fluid, especially a...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): G01N33/542C12M1/34A61K38/00B01F13/00B01L3/00C07K14/79C12N9/36G01N15/14G01N21/07G01N33/543G01N33/68G01N35/00
CPCA61K38/00Y10T436/111666B01F15/0233B01L3/5027B01L3/502715B01L3/50273B01L3/502738B01L7/00B01L2200/0605B01L2200/10B01L2300/021B01L2300/024B01L2300/069B01L2300/0806B01L2300/0864B01L2300/0867B01L2300/087B01L2300/0887B01L2300/168B01L2300/1805B01L2300/1861B01L2400/0406B01L2400/0409B01L2400/0661B01L2400/0677C07K14/79C12N9/2462G01N15/14G01N21/07G01N33/54366G01N33/569G01N33/68G01N35/00069Y10S366/03Y10T436/2575Y10T436/25375B01F13/0059B01F33/30B01F35/71725
Inventor SHEPPARD, NORMAN F. JR.MIAN, ALECKELLOGG, GREGORYKIEFFER-HIGGINS, STEPHEN G.CARVALHO, BRUCE L.
Owner TECAN TRADING AG
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