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Regulation of endogenous gene expression in cells using zinc finger proteins

a technology of endogenous gene expression and recombinant zinc finger protein, which is applied in the direction of fusion polypeptide, peptide/protein ingredients, vectors, etc., can solve the problems of not being able to demonstrate high target efficacy or high specificity in vivo, and the method is labor-intensive and labor-intensive. , to achieve the effect of modulating the expression of endogenous cellular genes

Inactive Publication Date: 2005-09-29
SANGAMO BIOSCIENCES INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0023] In one embodiment, expression of the endogenous cellular gene is inhibited by at least about 75%-100%. In another embodiment, the inhibition of gene expression prevents gene activation.
[0026] In one embodiment, expression of the endogenous cellular gene is activated to at least about 200-500%. In another embodiment, activation of gene expression prevents repression of gene expression.

Problems solved by technology

This method is labor intensive and does not address the physiological consequences of down-regulation of a target-gene.
These methods have to date proven to be generally insufficient for many applications and typically have not demonstrated either high target efficacy or high specificity in vivo.

Method used

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  • Regulation of endogenous gene expression in cells using zinc finger proteins
  • Regulation of endogenous gene expression in cells using zinc finger proteins
  • Regulation of endogenous gene expression in cells using zinc finger proteins

Examples

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examples

[0228] The following examples are provided by way of illustration only and not by way of limitation. Those of skill in the art will readily recognize a variety of noncritical parameters that could be changed or modified to yield essentially similar results.

example i

Design and Testing of ZFPs Targeted to the Human VEGF Gene

[0229] This first Example demonstrates the construction of ZFPs designed to recognize DNA sequences contained in the promoter of the human vascular endothelial growth factor (VEGF) gene. VEGF is an approximately 46 kDa glycoprotein that is an endothelial cell-specific mitogen induced by hypoxia. VEGF has been implicated in angiogenesis associated with cancer, various retinopathies, and other serious diseases. The DNA target site chosen was a region surrounding the transcription initiation site of the gene. The two 9 base pair (bp) sites chosen are found within the sequence agcGGGGAGGATcGCGGAGGCTtgg, where the upper-case letters represent actual 9-bp targets. The protein targeting the upstream 9-bp target was denoted VEGF1, and the protein targeting the downstream 9-bp target was denoted VEGF3a. The major start site of transcription for VEGF is at the T at the 3′ end of the first 9-bp target, which is underlined in the sequen...

example ii

Linking ZFPs to Bind an 18-bp Target in the Human VEGF Gene

[0248] An important consideration in ZFP design is DNA target length. For random DNA, a sequence of n nucleotides would be expected to occur once every 0.5×4n base-pairs. Thus, DNA-binding domains designed to recognize only 9 bp of DNA would find sites every 130,000 bp and could therefore bind to multiple locations in a complex genome (on the order of 20,000 sites in the human genome). 9-bp putative repressor-binding sequences have been chosen for VEGF in the 5′ UTR where they might directly interfere with transcription. However, in case zinc finger domains that recognize 9-bp sites lack the necessary affinity or specificity when expressed inside cells, a larger domain was constructed to recognize 18 base-pairs by joining separate three-finger domains with a linker sequence to form a six-finger protein. This should ensure that the repressor specifically targets the-appropriate sequence, particularly under conditions where o...

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Abstract

The present invention provides methods for modulating expression of endogenous cellular genes using recombinant zinc finger proteins.

Description

CROSS-REFERENCES TO RELATED APPLICATIONS [0001] This application is related to Townsend and Townsend and Crew docket number 019496-001800, U.S. Ser. No. ______, filed Jan. 12, 1999, herein incorporated by reference in its entirety.STATEMENT REGARDING FEDERALLY SPONSORED RESEARCH AND DEVELOPMENT [0002] This invention was made with government support under Grant No. 1 R43 DK52251-01, awarded by the National Institutes of Health. The government has certain rights in this invention.FIELD OF THE INVENTION [0003] The present invention provides methods for regulating gene expression of endogenous genes using recombinant zinc finger proteins. BACKGROUND OF THE INVENTION [0004] Many, perhaps most physiological and pathophysiological processes can be controlled by the selective up or down regulation of gene expression. If methods existed for gene expression control, pathologies could be treated. Examples include the inappropriate expression of proinflamatory cytokines in rheumatoid arthritis,...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61K31/711A61K35/76A61K38/16A61K48/00A61P7/00C12N15/09A61P7/06A61P9/00A61P9/10A61P11/00A61P17/06A61P19/02A61P21/00A61P21/04A61P25/28A61P27/02A61P29/00A61P31/04A61P31/10A61P31/12A61P31/18A61P35/00A61P37/00C07K14/46C07K14/47C07K14/52C12N5/10C12N9/64C12N15/12C12N15/63C12N15/67C12N15/82C12N15/85C12P21/04C12Q1/68
CPCA61K48/00G01N33/5091C07K14/47C07K14/52C07K2319/00C12N15/63C12N15/67C12N15/8216C12N15/85C12N2830/002C12N2830/005C12N2830/008C12N2830/85A01K2217/05C07K2319/71C07K2319/81A01K67/0275C12Q1/66C12Q1/6897A61K48/005A61P11/00A61P17/06A61P19/02A61P21/00A61P21/04A61P25/28A61P27/02A61P29/00A61P31/00A61P31/04A61P31/10A61P31/12A61P31/18A61P33/02A61P35/00A61P3/06A61P37/00A61P43/00A61P7/00A61P7/06A61P9/00A61P9/10
Inventor COX, GEORGECASE, CASEYEISENBERG, STEPHENJARVIS, ERICSPRATT, SHARON
Owner SANGAMO BIOSCIENCES INC
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