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Determination primer and method for yellow croaker in different population

A technology for large yellow croaker and population, which is applied to identification primers and identification fields of different populations of large yellow croaker to achieve the effect of rapid identification

Inactive Publication Date: 2007-05-23
XIAMEN UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0004] The object of the present invention is to address the deficiencies in the existing methods for detecting large yellow croaker populations, and to provide a method for simultaneously identifying three geographical populations of Daiqu, Fujian, Yuedong, and Naozhou, and four populations of large yellow croaker, including breeding groups. Primer

Method used

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  • Determination primer and method for yellow croaker in different population

Examples

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Embodiment 1

[0017] Select Naozhou large yellow croaker and cultured large yellow croaker, and adopt the phenol-chloroform extraction method (see Huang Peitang et al. translation "Molecular Cloning Experiment Guide" (third edition), Science Press, September 2002) to extract the DNA of large yellow croaker , design a synthetic primer 5'TGCCGCACTT-3', and amplify its genome, the amplification program is: 95°C 5min→(94°C 40sec→42.5°C 40sec→72°C 60sec)×35cycles→72°C 5min→4°C∞ ; The amplification result is electrophoresed, stained and compared with the map, and a 1875bp band appears in the map, then the DNA sample comes from the large yellow croaker breeding population; if there is a 1085bp band in the 1000-1500bp interval and another If there is a 1250bp band, the DNA sample comes from the wild large yellow croaker of Naozhou nationality. The total volume of the PCR amplification reaction system is 25uL, and its various components and final concentrations are: Buffer 10mM, dNTP 0.2mM, Mg 2+ ...

Embodiment 2

[0020] Similar to Example 1, the difference is that the large yellow croaker of the Daiqu family and the Fujian-Guangdong group are selected. If there is only one 1250bp band in the interval of 1000-1500bp when comparing the spectra, the DNA sample comes from the wild large yellow croaker of the Daiqu family. Yellow croaker; if there is only one band of 1085bp in the interval of 1000-1500bp, the DNA sample comes from wild large yellow croaker of Fujian-Guangdong ethnic group.

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Abstract

Identifying primers and method of different groups of large yellow croaker, relates to a kind of primer and large yellow croaker. The invention provides an identifying primer (5'-TGCCGCACTT-3') for identifying 3 geographical species of Daiqu, west Min-yue, and Naozhou, and cultivation species. The identifying method comprises the steps of: extracting large yellow croaker DNA with phenol / chloroform, constructing a PCR system (25uL) containing Buffer 10mM, dNTP 0.2mM, Mg2+ 1.5mM, primer 0.2mM, Taq 1U,large yellow croaker DNA 50ng, and water as balance, detecting the product with agarose gel electrophoresis, dyeing with ethidium bromide, observing with UV, taking a picture, and recording. Different species are identified through comparing the result of electrophoresis and the provided standard graph.

Description

technical field [0001] The invention relates to a primer and large yellow croaker, in particular to a primer and a method for identifying different populations of large yellow croaker. Background technique [0002] Large yellow croaker (Pseudosciaena crocea (Richardson)), also known as yellow croaker, cucumber, and yellow croaker, belongs to Perciformes (Perciformes), Percoidei (Percoidei), Sceanidae (Scieanidae), Pseudosciaena (Pseudosciaena), used to be One of my country's famous "four major marine economic fish", its output is second only to hairtail and pelagic fish. It is distributed from the south of the Shandong Peninsula in the Yellow Sea to the east of the Leizhou Peninsula in the South China Sea. In the 1950s and 1960s, Chinese scientists conducted detailed studies on the division of large yellow croaker geographical populations (local ethnic groups). According to the number of gill rakers, the number of side branches of the swim bladder, and the height of the caud...

Claims

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Application Information

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IPC IPC(8): C12Q1/68G01N27/26
Inventor 王德祥王军苏永全
Owner XIAMEN UNIV
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