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Near infrared spectrum quick detecting technique for E.coli

A technology of near-infrared spectroscopy and coliform bacteria, applied in the field of detection technology, can solve the problems of laborious and long detection time, and achieve the effect of no pollution and short detection time

Inactive Publication Date: 2007-01-24
HENAN UNIV OF SCI & TECH
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Problems solved by technology

[0002] At present, when health and epidemic prevention departments and technical supervision and inspection departments measure food harmful microbiological indicators (such as the total number of bacteria, total coliforms, aflatoxin, etc.), they use the method of sampling first, then culturing, and then manually counting and calculating through a microscope In this way, it takes 2 days to complete the measurement of a sample, which not only takes a long time to detect, but also requires a lot of labor and effort

Method used

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Embodiment Construction

[0028] When testing the sample, first turn on the Fourier transform infrared spectrometer and preheat it for 20 minutes. After the instrument works normally, set the parameters of the instrument: the scanning accuracy of the spectrometer is 4cm -1 , the number of scans is 32 times, and the scanning spectrum range is 3000 -1 ~9000cm -1 ; The seven characteristic absorption peaks during near-infrared spectrum detection are: 7144, 3167, 3742, 6712, 3456, 6631, 5339; select near-infrared spectrum mode in the parameter setting menu; integrating sphere detection optical path; diffuse reflection mode. Perform a background scan according to the parameters set above, obtain the background scan spectrum, and store it in the computer; take 30ml of the sample to be tested and put it into a 50ml small beaker that has been cleaned, sterilized, and dried with distilled water; put the sample to be tested The small beaker is placed on the integrating sphere window of the Fourier transform in...

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Abstract

Based on spectroscopic analysis technique of Fourier transform infrared (FTIR), using near infrared spectral scan obtains near infrared spectroscopic data and spectral graph in range of wave number between 3000cm-1 and 9000cm-1. In detection of near infrared spectrum, seven characteristic absorption peaks are 7144, 3167, 3742, 6712, 3456, 6631, 5339. The invention is rapid analysis method of carrying out quantitative analysis or discrimination for components of matter. Using interaction between infrared radiation and molecular vibration or rotation, recording infrared absorption spectrum of sample, the disclosed method carries out qualitative, quantitative and structural analysis. Features are: not destroying object to be tested, short test time, and no pollution.

Description

Technical field: [0001] The invention relates to a detection technology, in particular to a rapid detection technology of near-infrared spectrum of coliform bacteria. Background technique: [0002] At present, when health and epidemic prevention departments and technical supervision and inspection departments measure food harmful microbiological indicators (such as the total number of bacteria, total coliforms, aflatoxin, etc.), they use the method of sampling first, then culturing, and then manually counting and calculating through a microscope In this way, it takes 2 days to complete the measurement of a sample, which not only takes a long time to detect, but also takes a lot of work. [0003] Chinese application (patent) number retrieved: 00134390.4 Application date: 2000.12.12 Publication (announcement) number: CN 1295243 Publication (announcement) date: 2001.05.16 [0004] Application (patent) number: 01137316.4 Application date: 2001.11.28 Publication (announcement) n...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/35C12Q1/10G01N21/3563G01N21/3577
Inventor 刘建学李市场唐浩国纠敏殷勇侯玉泽
Owner HENAN UNIV OF SCI & TECH
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