Modified skin tinea fungus test culture medium
A dermatophyte and culture medium technology, which is applied in the field of fungal chromogenic medium, can solve the problems of different antifungal drug spectrums, results judgment standards need to be improved, antibiotics are not resistant to high temperature, etc., and the reporting time can be advanced and accurate The effect of high efficiency and formula economy
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0020] 1. Medium preparation:
[0021] Element
[0022] Soy peptone 5g
[0023] Glucose 5g
[0024] Chloramphenicol 0.0625g
[0025] Cycloheximide 0.1g
[0026] 0.1% bromothymol blue 25mL
[0027] Agar 18g
[0028] HCL(1.0N) 4.6-5.0mL
[0029] Add water to 1000mL
[0030] pH5.5±0.1
[0031] Preparation method: Add soybean peptone, glucose and agar to water, heat to dissolve; add bromothymol blue solution while stirring, adjust pH with 1N Hcl; add cycloheximide and chloramphenicol to medium; 121°C Autoclave for 10 minutes.
[0032] 2. Results report:
[0033] Specimens were inoculated according to the conventional fungal culture method, and the discoloration of the medium and the growth of the strains were regularly observed every day. If the colony diameter was ≤5mm when the discoloration began, there was a 95% possibility of being a dermatophyte, otherwise it was a non-dermatophyte.
Embodiment 2
[0035] 1. Medium preparation:
[0036] Element
[0037] Soy peptone 5g
[0038] Glucose 5g
[0039] Chloramphenicol 0.125g
[0040] Cycloheximide 0.3g
[0041] 0.1% bromothymol blue 50mL
[0042] Agar 19g
[0043] HCL(1.0N) 4.6-5.0mL
[0044] Add water to 1000mL
[0045] pH5.5±0.1
[0046] Preparation method: Add soybean peptone, glucose and agar to water, heat to dissolve; add bromothymol blue solution while stirring, adjust pH with 1N Hcl; add cycloheximide and chloramphenicol to medium; 121°C Autoclave for 10 minutes.
[0047] 2. Results report:
[0048] Specimens were inoculated according to the conventional fungal culture method, and the discoloration of the medium and the growth of the strains were regularly observed every day. If the colony diameter was ≤5mm when the discoloration began, there was a 95% possibility of being a dermatophyte, otherwise it was a non-dermatophyte.
Embodiment 3
[0050] 1. Medium preparation:
[0051] Element
[0052] Soy peptone 5g
[0053] Glucose 5g
[0054] Chloramphenicol 0.25g
[0055] Cycloheximide 0.5g
[0056] 0.1% bromothymol blue 100mL
[0057] Agar 20g
[0058] HCL(1.0N) 4.6-5.0mL
[0059] Add water to 1000mL
[0060] pH5.5±0.1
[0061] Preparation method: Add soybean peptone, glucose and agar to water, heat to dissolve; while stirring, add bromothymol blue solution, adjust pH with 1N HCL; add cycloheximide and chloramphenicol to medium; 121°C Autoclave for 10 minutes.
[0062] 2. Results report:
[0063] Specimens were inoculated according to the conventional fungal culture method, and the discoloration of the medium and the growth of the strains were regularly observed every day. If the colony diameter was ≤5mm when the discoloration began, there was a 95% possibility of being a dermatophyte, otherwise it was a non-dermatophyte.
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com