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Modified skin tinea fungus test culture medium

A dermatophyte and culture medium technology, which is applied in the field of fungal chromogenic medium, can solve the problems of different antifungal drug spectrums, results judgment standards need to be improved, antibiotics are not resistant to high temperature, etc., and the reporting time can be advanced and accurate The effect of high efficiency and formula economy

Inactive Publication Date: 2007-01-24
CHUGOKU IGAKU KAGAKUIN HIFUBIYOU KENKYUSHO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] DTM medium has high practical value because: 1. In recent years, the incidence of fungal infection has been increasing year by year, and the diagnosis and treatment of such diseases is the focus of attention of both government health departments and doctors and patients; 2. Different antifungal drugs The antimicrobial spectrum is not the same, some fungal infectious diseases such as onychomycosis have a long course of treatment and high cost, and the drugs have potential adverse reactions, so the identification of bacterial species and targeted and reasonable treatment are very important for such diseases It is especially important; 3. The traditional fungal culture identification method has high requirements on the experience and skills of the inspectors. At present, only a few hospitals in China have carried out this project, and it generally takes about 2 weeks to culture, and DTM medium produces positive results. In less than a week, the results are intuitive and can be reported by non-professionals
2. The result judgment standard needs to be improved
3. The antibiotic (chlortetracycline) in the medium is not resistant to high temperature and needs to be added after autoclaving, which is relatively cumbersome

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 1. Medium preparation:

[0021] Element

[0022] Soy peptone 5g

[0023] Glucose 5g

[0024] Chloramphenicol 0.0625g

[0025] Cycloheximide 0.1g

[0026] 0.1% bromothymol blue 25mL

[0027] Agar 18g

[0028] HCL(1.0N) 4.6-5.0mL

[0029] Add water to 1000mL

[0030] pH5.5±0.1

[0031] Preparation method: Add soybean peptone, glucose and agar to water, heat to dissolve; add bromothymol blue solution while stirring, adjust pH with 1N Hcl; add cycloheximide and chloramphenicol to medium; 121°C Autoclave for 10 minutes.

[0032] 2. Results report:

[0033] Specimens were inoculated according to the conventional fungal culture method, and the discoloration of the medium and the growth of the strains were regularly observed every day. If the colony diameter was ≤5mm when the discoloration began, there was a 95% possibility of being a dermatophyte, otherwise it was a non-dermatophyte.

Embodiment 2

[0035] 1. Medium preparation:

[0036] Element

[0037] Soy peptone 5g

[0038] Glucose 5g

[0039] Chloramphenicol 0.125g

[0040] Cycloheximide 0.3g

[0041] 0.1% bromothymol blue 50mL

[0042] Agar 19g

[0043] HCL(1.0N) 4.6-5.0mL

[0044] Add water to 1000mL

[0045] pH5.5±0.1

[0046] Preparation method: Add soybean peptone, glucose and agar to water, heat to dissolve; add bromothymol blue solution while stirring, adjust pH with 1N Hcl; add cycloheximide and chloramphenicol to medium; 121°C Autoclave for 10 minutes.

[0047] 2. Results report:

[0048] Specimens were inoculated according to the conventional fungal culture method, and the discoloration of the medium and the growth of the strains were regularly observed every day. If the colony diameter was ≤5mm when the discoloration began, there was a 95% possibility of being a dermatophyte, otherwise it was a non-dermatophyte.

Embodiment 3

[0050] 1. Medium preparation:

[0051] Element

[0052] Soy peptone 5g

[0053] Glucose 5g

[0054] Chloramphenicol 0.25g

[0055] Cycloheximide 0.5g

[0056] 0.1% bromothymol blue 100mL

[0057] Agar 20g

[0058] HCL(1.0N) 4.6-5.0mL

[0059] Add water to 1000mL

[0060] pH5.5±0.1

[0061] Preparation method: Add soybean peptone, glucose and agar to water, heat to dissolve; while stirring, add bromothymol blue solution, adjust pH with 1N HCL; add cycloheximide and chloramphenicol to medium; 121°C Autoclave for 10 minutes.

[0062] 2. Results report:

[0063] Specimens were inoculated according to the conventional fungal culture method, and the discoloration of the medium and the growth of the strains were regularly observed every day. If the colony diameter was ≤5mm when the discoloration began, there was a 95% possibility of being a dermatophyte, otherwise it was a non-dermatophyte.

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PUM

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Abstract

The modified dermatophyte test culture medium is compounded with soybean peptone 5g / L, glucose 5 g / L, chloromycetin 0.0625-0.25g / L, cycloheximide 0.1-0.5g / L, bromthymol blue 0.025-0.1g / L, agar 20g / L, and 1N HCl 4.8mL, and has pH value of 5.5 + / - 0.1. The present invention has the advantages of capacity of resulting in accurate interpretation, short culture period and low cost, except the advantages of traditional DTM culture medium. The present invention has latent clinical application foreground.

Description

technical field [0001] The invention relates to a fungal chromogenic medium, in particular to an improved dermatophyte test medium capable of rapidly distinguishing dermatophyte from non-dermatophyte fungi. Background technique [0002] Dermatophytes are a large group of fungi that can cause infections of the skin, hair, and nail plate in humans and animals. The incidence of dermatophytosis is very high, and the total number of patients in my country is estimated to be as high as hundreds of millions. Before clinical treatment, doctors often have to conduct fungal tests for patients. Only when it is clear that the infection is fungal and what kind of fungal infection is it, can effective antifungal drugs be selected for thorough treatment. Fungal culture is the "gold standard" for diagnosis. One of the key factors affecting the speed and accuracy of fungal culture is the culture medium. [0003] nitrogen source carbon source antibiotics Cycloheximide ...

Claims

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Application Information

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IPC IPC(8): C12P1/04
Inventor 刘维达李筱芳沈永年吕桂霞陈伟
Owner CHUGOKU IGAKU KAGAKUIN HIFUBIYOU KENKYUSHO
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