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Hypoglycemic polypeptide from silkworm and its prepn and use

A technology of glycosidase inhibition and glucose, which is applied in the fields of chemistry and biology, can solve the problems of no hypoglycemic effect found and no elaboration

Inactive Publication Date: 2007-01-03
上海国源生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many substances in silkworm powder, such as fat, sugar, protein, inorganic salts, vitamins, hormones, polypeptides, pigments, etc. The mechanism of action has not been elucidated in detail
Some scholars have extracted some small molecular peptides from silkworm powder. However, after injecting these small molecular peptides, they have not found the hypoglycemic effect like insulin.

Method used

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  • Hypoglycemic polypeptide from silkworm and its prepn and use
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  • Hypoglycemic polypeptide from silkworm and its prepn and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1A

[0079] Preparation of Silkworm Hypoglycemic Polypeptide

[0080] Take 5 grams of fresh silkworm pupae and crush them at 4°C to homogenize, add 3 times the volume of sterile distilled water, stir at 4°C for 2 hours. After mixing thoroughly, centrifuge at 10000×g for 15 minutes to remove the precipitate, and take the supernatant for separation and purification.

[0081] Take an appropriate amount of supernatant and apply it to the column for separation. The CM Sepharose FF filler produced by Pharmacia was used, and the buffer was a pH 5.8 HAc-NaAc system, and the linear gradient eluted to the buffer containing 1M NaCl. The specific separation and purification conditions are as follows:

[0082] 1. Column type: inner diameter 1.6cm, column height 20cm, column bed volume is 30ml.

[0083] 2. Packing: CM Sepharose FF, produced by Pharmacia.

[0084] 3. Sample loading: the supernatant after centrifugation.

[0085] 4. Column equilibration conditions: 20mM Tris buffer, pH 7.1, equilibrat...

Embodiment 1B

[0098]Preparation of Silkworm Hypoglycemic Polypeptide

[0099] Example 1A was repeated, but the difference was that 5 grams of fresh silkworm fifth instar larvae were used to replace the silkworm pupa in Example 1A.

[0100] The result was basically the same as that of Example 1A, and there was a main peak of silkworm hypoglycemic polypeptide eluting at a concentration of 0.230-0.255M NaCl. The amount of the prepared silkworm hypoglycemic polypeptide is 0.298 g, which is about 80-90% of that in Example 1A.

Embodiment 1C

[0102] Preparation of Silkworm Hypoglycemic Polypeptide

[0103] Repeat Example 1A, the difference is that 1 gram of freeze-dried silkworm powder from the fifth-instar larva of the silkworm was used to replace the silkworm pupae in Example 1A. The freeze-dried silkworm powder was first dissolved in 3 kg of sterile distilled water and stirred at 4°C. 2 hours. After mixing thoroughly, centrifuge at 10000×g for 15 minutes to remove the precipitate, and take the supernatant for separation and purification.

[0104] The result was basically the same as that of Example 1A, and there was a main peak of silkworm hypoglycemic polypeptide eluting at 0.230-0.255M NaCl concentration. The amount of the prepared silkworm hypoglycemic polypeptide was 0.375 grams.

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Abstract

The present invention discloses one kind of hypoglycemic polypeptide from silkworm and its preparation process and use as well as composition, medicine, food, tonic and health product connecting the hypoglycemic polypeptide. The hypoglycemic polypeptide of the present invention can combine with glycosidase inhibitor in intestinal tract competitively to inhibit the activity of glycosidase partially, lower the saccharide decomposing speed of glycosidase, lower the amount of glucose produced in unit time and lower the sugar absorbing amount unit time, reaching hypoglycemic effect.

Description

Technical field [0001] The invention belongs to the fields of chemistry and biology. More specifically, the present invention relates to a silkworm-derived polypeptide with hypoglycemic effect (referred to as "Silkworm Hypoglycemic Polypeptide"), its preparation method and use, and also relates to a composition, medicine, and drug containing the silkworm hypoglycemic polypeptide. Food, dietary supplements and health products. Background technique [0002] Silkworms, such as Bombyx mori, are important economic insects, mainly used to extract their silk cocoons, and are the main silk raw materials in the textile industry. At the same time, silkworm is also a kind of medicinal economic insects. Stiff silkworm, silkworm litter, silkworm slough, silkworm pupa can be used as medicine to treat diseases such as low immunity. [0003] Korean scientists have used freeze-dried silkworm powder to test the effect of reducing blood sugar. Chinese scholar Zhuang Daheng and others also used whol...

Claims

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Application Information

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IPC IPC(8): C07K14/435A61K38/17A61P3/10A23L1/305A23L33/18
Inventor 黄文华
Owner 上海国源生物技术有限公司
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