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High-yield twin-corn plantation and its cenospecies selective breeding method

A technology of planting method and preparation method, which is applied in the fields of botany equipment and method, plant gene improvement, application, etc., can solve the problems of cumbersome breeding procedures, breeding of sterile hybrids that have not been reported, etc., so as to simplify the breeding procedure Effect

Inactive Publication Date: 2006-12-27
INST OF CEREAL & OIL CROPS HEBEI ACAD OF AGRI & FORESTRY SCI
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Problems solved by technology

[0003] Sterile hybrids are a new type of hybrids that have been studied more in recent years, but the breeding of sterile hybrids has not been reported so far
Patents such as 200510012523.9 and 200510012669.3 disclose a method for producing male sterile corn hybrids, but the so-called male sterile corn hybrids are fertile. The phenomenon of inbreeding depression has not been avoided in large-scale planting in production; and this method requires the cultivation of restorer lines in addition to the cultivation of sterile lines and maintainer lines, so the breeding procedure is relatively cumbersome

Method used

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  • High-yield twin-corn plantation and its cenospecies selective breeding method

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Experimental program
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Embodiment 1

[0020] Embodiment 1: the breeding preparation of hybrid

[0021] ①Transfer of the sterile gene: In 2000, the female parent inbred line Ji 15-22 of Jiyu 9 (its hybrid combination is Ji 15-22×Ji 35) was used as the male parent, and the C-type Bb subspecies was sterile The inbred line Mo17 was crossed with the female parent to obtain the C-type Bb subspecies sterile F 1 seed;

[0022] ②Backcross: F bred in the planting step ① in Hainan in the winter of 2000 1 For generation seeds, all fertile plants were pulled out at the tasseling stage, male sterile plants were selected, and Ji 15-22 was used as the male parent for backcrossing, and among the backcrossing plants, the plants with excellent agronomic characteristics and similar to Ji 15-22 were selected to retain fruit ears. species, and obtained sterile Bb Ji 15-22BC including 5 sister lines 1 Seeds, pay attention to sisters to be harvested separately;

[0023] ③Continuous backcrossing: Planting in the summer of 2001 Step ②M...

Embodiment 2

[0026] Example 2: Planting of high-yielding mandarin duck corn

[0027] Utilize the mandarin duck hybrids selected in step ⑤ of embodiment 1, carry out interplanting in the field according to the ratio of Bb type Jiyu No. 9 sterile hybrids and fertile hybrids Jiyu No. 12 with a row ratio of 4:1, and the planting density is 4000 Plant / mu, 741.1 kg of corn per mu, 22.88% higher than that of Jiyu No. 9, a conventional single-cross corn planted under the same conditions.

[0028] The Bb-type sterile hybrid Jiyu 9 has the same characteristics as the conventional maize single-cross Jiyu 9 except for fertility.

Embodiment 3

[0029] Embodiment 3: the breeding preparation of hybrid

[0030] The transfer of the sterile gene in step ① of Example 1 is a hybridization of the C-type Es subspecies sterile inbred line Huangzaosi as the female parent to obtain the C-type Es subspecies sterile F 1 Seed; the fertile hybrid used in the combining ability test in step 5 of Example 1 is Zhengdan 958, and the Es type Jiyu No. 9 sterile hybrid and Zhengdan 958 are mandarin duck hybrids; the rest are the same as in Example 1.

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Abstract

The invention relates to a method for planting high-yield mandarin duck maize, and hybrid seed selection and preparation method thereof, characterized in: inseminating sterile hybrid seeds and normal hybrid seeds together, the sterile seeds are selected and bred successfully by the following step: (1) transforming sterile genes; (2) backcrossing; (3) backcrossing again; (4) arranging different hybrid seeds in one group; (5) GCA test. Yield of planting mandarin duck maize hybrid seeds produced by this method is increased by 14-22.88% than that of planting normal single cross hybrid maize seeds. Only male sterility line is needed to be cultivated, and neither does restoring line, which simplifies the breeding program of maize, saves time and labour.

Description

technical field [0001] The invention relates to a method for planting high-yield mandarin duck corn and hybrid breeding, in particular to a method for breeding sterile corn hybrids and planting mandarin duck corn, which belongs to the utilization of hybrid vigor, male sterility and pollen intuition effect Research areas. Background technique [0002] Maize is a high-yield crop with the most obvious economic benefits and the greatest potential for yield increase through the use of hybrid vigor. 1 The hybrid vigor of the plant, because the commercial grain harvested is F 2 seeds, so there is a certain phenomenon of inbreeding depression (Duan Minxiao et al., 2001). [0003] Sterile hybrids are a new type of hybrids that have been studied more in recent years, but the breeding of sterile hybrids has not been reported so far. Patents such as 200510012523.9 and 200510012669.3 disclose a method for producing male sterile corn hybrids, but the so-called male sterile corn hybrids...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/00A01H1/02A01H1/04A01G1/00
Inventor 张文英郑积德李海军王宝强张动敏宋炜陈玲
Owner INST OF CEREAL & OIL CROPS HEBEI ACAD OF AGRI & FORESTRY SCI
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