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Method for detecting bifidobacteria in milk product

A bifidobacteria and detection method technology, applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of non-identification, increased counting workload, and difficulty in the detection of bifidobacteria, and achieve Good discrimination and reduced counting workload

Active Publication Date: 2006-12-20
WANDASHAN MILK IND HEILONGJIANG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The special physiological and health functions of bifidobacteria are well known. Many probiotic products have been developed abroad, but such products have not yet reached the level of popularization in China. With the continuous increase in the types and quantities of bifidobacteria dairy products Increase, many issues related to bifidobacteria products should be regulated and programmed, and there are still no clear regulations on the intake of bifidobacteria, the number of viable bacteria and the detection methods of bacteria counts, especially the determination of bifidobacteria in mixed fermented dairy products is very difficult. difficulty
[0005] "GB / T 16347-1997" only stipulates the detection method of the viable count of lactic acid bacteria. In 2004, the state issued the detection method of bifidobacteria "GB / T 4789.34-2003". In this standard, non-selective medium is used. Counting, although this method can realize the detection of live bacteria of bifidobacteria, it is not very distinguishable. Streptococcus, lactobacillus and other types of bacteria will grow and proliferate on it, and further colony identification is required when counting. Increased counting workload

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0074] Sample to be tested: known number of viable bifidobacteria (5×10 11 cfu / g) milk powder sample

[0075] Preparation of the sample: dry mix the Bifidobacterium powder with known viable count with the Lactobacillus acidophilus powder in a certain proportion, add it to the milk powder, and wait for the test.

[0076] Prepare BL medium according to the formula: tryptone 5g, peptone 5g, plantptone 3g, yeast powder 2g, glucose 10g, soluble starch 0.5g, beef extract 3g, Tween 80 1.0ml, A solution 10ml, B solution 5ml, liver extract Liquid 150ml, agar 20g, 5% L-cysteine ​​hydrochloride 10ml, distilled water to 1000mL.

[0077] Liquid A: 10 g of potassium dihydrogen phosphate, 10 g of dipotassium hydrogen phosphate, dilute to 100 ml; liquid B: 10 g of magnesium sulfate, 0.5 g of ferric sulfate, 0.5 g of sodium chloride, 0.337 g of manganese sulfate, dilute to 250 ml.

[0078] Adjust the pH to 7.2±0.1; sterilize at 115°C for 20 minutes. Liquid A and liquid B were sterilized for...

Embodiment 2

[0086] Sample to be tested: known number of viable bifidobacteria (5×10 11 cfu / g) milk powder sample

[0087] Preparation of the sample: dry-mix the Bifidobacterium powder with the known number of viable bacteria with the Streptococcus thermophilus powder in a certain proportion, add it to the milk powder, and wait for the test.

[0088] Prepare BBL medium according to the formula: peptone 15g, yeast powder 2g, glucose 20g, soluble starch 0.5g, sodium chloride 5g, 5% L-cysteine ​​hydrochloric acid 10ml, tomato extract 400ml, liver extract 80ml, Tween 80 1ml, 20g agar, and distilled water to make up to 1000mL. Adjust the pH to 7.1, sterilize at 115°C for 15 minutes.

[0089] Add antibiotics to the medium, per 100ml liquid medium:

[0090] 30% lithium chloride 1.0ml

[0091] 30% sodium propionate 3.0ml

[0092] 10% dicloxacillin 0.5ml.

[0093] According to GB / T 4789.34-2003 Bifidobacteria detection operation steps and strain identification method for detection.

[0094] ...

Embodiment 3

[0096] Sample to be tested: yogurt sample prepared in the laboratory

[0097] Prepare TPY medium according to the formula: trypticase casein 10g, soybean peptone 5g, glucose 5g, fructooligosaccharide 5g, yeast powder 5g, Tween-80 1g, K 2 HPO 4 2g, MgCl 2 ·6HO 2 0.5g, ZnSO 4 ·7HO 2 0.25g, FeCl 3 0.05g, CaCl 2 0.15g, tomato juice 200mL, L-cysteine ​​hydrochloride 0.5g, dilute to 1000mL, adjust pH=7.2, sterilize at 115°C for 15-20min.

[0098] Add antibiotics to the medium, per 100ml liquid medium:

[0099] 30% lithium chloride 1.5ml

[0100] 30% sodium propionate 3.0ml

[0101] 10% dicloxacillin 0.7ml

[0102] According to GB / T 4789.34-2003 Bifidobacteria detection operation steps and strain identification method for detection.

[0103] Results: The number of viable bifidobacteria was 2.6×10 9 cfu / g.

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Abstract

The invention discloses a bifidobacteria detecting method in the milk product, which is characterized by the following: adding antibiotics in the culture base to inhibit growing other bacteria; detecting bifidobacteria selectively; studying lithium chloride, sodium propionate and maclicine to obtain fittest antibiotics proportion and quantity of bifidobacteria selectivity count.

Description

technical field [0001] The invention belongs to the field of food detection, and in particular relates to a method for detecting bifidobacteria in milk powder or yoghurt. Background technique [0002] Bifidobacterium is one of the earliest physiological bacteria discovered. Since it was first isolated by Dr. Tissier in 1899, the research on Bifidobacterium has been paid more and more attention by scholars from all over the world. Bifidobacteria have a series of physiological functions such as regulating intestinal flora, lowering cholesterol, anti-tumor, regulating immunity, and synthesizing vitamins, which are of great significance to human health. Bifidobacteria have special physiological functions and health effects, so they have become commonly used probiotics in microecological health foods. [0003] Research on the development and application of bifidobacterium products began in the mid-1950s, when the Japanese Institute of Biological Sciences first developed bifidoba...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04
Inventor 焦发道徐敬华夏元军孟祥晨吕文君王占东轩诗海
Owner WANDASHAN MILK IND HEILONGJIANG
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