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Methods and compositions for analysis of urine samples in the diagnosis and treatment of kidney diseases

A kidney disease, sample technology, applied in the field of analysis of biological samples in the diagnosis and treatment of kidney disease

Inactive Publication Date: 2006-11-15
RGT UNIV OF MICHIGAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this method is not as sensitive as our presented method because it is only abnormal in the presence of albumin in the urine

Method used

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  • Methods and compositions for analysis of urine samples in the diagnosis and treatment of kidney diseases
  • Methods and compositions for analysis of urine samples in the diagnosis and treatment of kidney diseases
  • Methods and compositions for analysis of urine samples in the diagnosis and treatment of kidney diseases

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Embodiment

[0029] RNA isolation. All urine samples were collected with informed consent. Samples consisted of approximately 3-40 mL of spontaneously voided urine. The urine used for this purpose was either fresh or previously frozen. Urine was centrifuged at 8000 rpm for 10 minutes. Decant the supernatant, and use pH7.4 phosphate buffered saline (137mM NaCl+2.7mM KCl+4.3mM NaCl 2 HPO 4 +1.4mM KH 2 PO 4 ) to wash the pellet once, then centrifuge again, resuspend the air-dried pellet in a small amount of double distilled water, aliquot and freeze at -80°C.

[0030] The invention is used to detect RNA in urine precipitated cells. Urine is stored in the bladder for several hours in the body at about 37°C before being passed out of the body. The urine is then usually left out at room temperature in the clinic for several hours. Urine pellets were then centrifuged and treated with RNAlater (Ambion) or STG buffer (Biotronics, Lowell MA) to inactivate RNases. If the sample is obtained ...

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Abstract

The invention comprises novel compositions, systems, and methods for the analysis of biological samples, such as urine samples, in order to diagnose and treat kidney disease, including without limitation, glomerulonephritis, nephrotic syndrome, diabetes, lupus, hypertension, acute tubular necrosis, obstructive disorders of the urinary tract, renal cancers and other diseases or syndromes. In preferred embodiments, the invention comprises the identification of particular genes expressed only in cells of particular clinical or scientific interest in the urinary tract, such as podocytes or proximal tubule cells. Thus, the invention enables non-invasive, rapid and accurate analysis of kidney and urinary tract status and function in many medical diseases and disorders.

Description

[0001] Part of the work of the present invention is carried out under the support of the US government NIH RO1 CA78853 document. The government has certain rights in this invention. field of invention [0002] The present invention relates to the analysis of biological samples in the diagnosis and treatment of renal diseases. In particular, the present invention relates to the diagnosis and monitoring of kidney disease and its relationship to various conditions including but not limited to glomerulonephritis, nephrotic syndrome, diabetes mellitus, lupus, hypertension, acute tubular necrosis (ATN) by analysis of urine samples , renal obstructive disease, renal cancer) and the progress of treatment of other diseases or syndromes. Background of the invention [0003] Physicians often need to analyze kidney status and function when treating patients. For diseases such as glomeruli, this is currently achieved by detecting protein (albumin) in the urine, ie measuring the amount ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68G01N33/493A61KA61K6/00C07H21/04C12N15/09C12P19/34C12QC12Q1/02G01N33/48G01N33/68
CPCC12Q1/6881C12Q1/6883C12Q2600/158
Inventor D·M·库尔尼特
Owner RGT UNIV OF MICHIGAN
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