Method for selecting and breeding corn new-bred through hybridized induction of unisexual seeding material
A technology of parthenocarpy and hybridization, which is applied in the direction of botany equipment and methods, application, plant gene improvement, etc., to achieve the effects of good comprehensive agronomic traits, easy identification, and eliminating the process of self-purification
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Embodiment 1
[0026] In 2004, the foreign hybrid X1132P with high yield and excellent comprehensive traits was pollinated by using the parthenogenic induction line Jigaoyin Line 3 as the male parent. Normal hybrid diploid grains; while the aleurone layer at the top is purple, the germ tip is colorless, and the germ surface is small, triangular in shape, and deeply depressed, it is the "haploid" X1132P parthenocarpy induced "Grains; A total of 1023 "haploid" grains were obtained according to the above markers.
[0027] In 2005, the selected "haploid" grains were carefully sown, and 693 plants emerged. The jointing stage was further confirmed according to the growth of the seedlings, plant height, leaf length, leaf sheath (sheet) color, and leaf angle. The haploid seedlings grow slowly, the plant height is low, the plants are thin, the leaves are short and relatively upward, and the leaf color is light; the diploid seedlings grow strong, the plants are tall and the leaves are hypertrophic, an...
Embodiment 2
[0030] In 2003, the “Huangzao Four Groups” was selected as the backbone germplasm of domestic maize (in 2001, there were 13 Huangzao four populations including j853, Chang7-2, Si444, 773-2, K12, K14, Zhe461, H23, etc. The population synthesized by cross-line artificial mixing) is the basic material for line selection, and the pollen of No. 3 Jigaoyu line is used to pollinate it. Marker traits such as triangle shape and deep depression were identified, and 732 "haploid" grains were obtained.
[0031] In 2004, the selected "haploid" seeds were sown finely, and 471 plants emerged. At the jointing stage, 423 haploid plants were further confirmed according to the growth of seedlings, plant height, leaf length, and leaf color. 36 diploid or double haploid plants were obtained through natural doubling, and 13 self-fertilized fruit ears.
[0032]In the winter of 2005, three new inbred lines were added in Hainan, and the selected offspring showed uniform performance and good comprehen...
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