Method for separating and purifying phycobiliprotein in high purity from laver

Abstract

This invention relates to an extraction method for high-purity phycobiliprotein from lavers. Currently, phycobiliprotein extraction from spirulina suffers some technological drawbacks, which causes the high prices for high-purity phycobiliprotein as a result of commonly low extraction purities and yields. In contrast, it is main characteristics of this invention that phycobiliprotein is extractedfrom lavers, which includes following steps: lavers are pounded into pieces at a low temperature and a high speed and the mixture is centrifugalized to obtain the supernatant; original phycobiliprotein extract is prepared by multiple ammonium sulphate salting-out; it is then dialyzed and centrifugalized at a high speed with the obtained solution placed unto a hydroxyapatite column; stepwise low-concentration elution is implemented at first to obtain phycoerythrin and phycocyanin respectively and high-concentration is then implemented to obtain allophycocyanin. Generally speaking, the extraction method for high-purity phycobiliprotein from lavers introduced in this invention has the advantages of simple technique, and practicability.

Description

Technical field: [0001] The invention relates to a method for separating and purifying high-purity phycobiliprotein. In particular, it relates to a method for separating and purifying high-purity phycobiliprotein from laver. Background technique: [0002] Phycoerythrin, phycocyanin, and variable phycocyanin are good pure natural pigments with bright colors, which can be used as food pigments and also in the cosmetics industry. They will not cause artificial harm and are ideal and safe Additives. At the same time, phycoerythrin, phycocyanin, and variable phycocyanin are also active substances, all of which have stable fluorescence and generate free radicals. They can be used as antibody fluorescent labeling substances to replace synthetic fluorescent substances. Therefore, phycocyanin has been produced. Protein and phycoerythrin fluorescently labeled immune antibody reagents are used for clinical molecular diagnosis of medical diseases and life science molecular detection. I...

AI Technical Summary

Problems solved by technology

At present, phycobiliproteins can be extracted from several species of marine red algae, but the price of high-purity phycobiliproteins is very expensive, and the price is still rising rapidly.

[0003] Regarding the extraction and purification of phycobiliproteins, although the methods are different, most of them extract phycocyanin from spirulina. Moreover, some processes are simple, and the purity of the protein obtained is extremely low, and some processes are too complicated, and it is easy to make the protein Denaturation, not suitable for large-scale extraction, and some food stabilizers, sugars, inorganic salts, pH regulators, etc. are added during the extraction process, generally only pharmaceutical grade (A 615nm / A 280nm >2) phycocyanin, it is difficult to reach reagent grade (A 615nm / A 280nm >4), it is precisely because of these defects in the above-mentioned separation techniques that the purity of the obtained phycobiliprotein is low and the yield is not high, so that the price of high-purity phycobiliprotein is high, and finally, it greatly affects the high-purity phycobiliprotein. Phycobiliproteins are widely used, therefore, reducing the cost of phycobiliprotein purification is the key