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Process of separating fucoxanthin from algae

A fucoxanthin and seaweed technology, applied in the direction of organic chemistry, can solve the problems of high alginate and polysaccharide content, difficulty in extraction, time-consuming and labor-intensive work, and achieve fast separation speed, low cost and good separation effect

Inactive Publication Date: 2005-12-14
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] (1) Time-consuming and labor-intensive, the extraction process needs to be ground, so the large algae must be cut into pieces, and the pigment and residue must be centrifuged or filtered through gauze
[0010] (2) Due to the large content of alginate and polysaccharide in large brown algae such as kelp, it brings great difficulty to the extraction work
[0011] (3) The yield is low, and acetone extraction is used to extract a large amount of chlorophyll a[chl a] and chlorophyll c[chl c] at the same time

Method used

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  • Process of separating fucoxanthin from algae
  • Process of separating fucoxanthin from algae
  • Process of separating fucoxanthin from algae

Examples

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Comparison scheme
Effect test

Embodiment 1

[0037] Take out the fresh or frozen kelp root, thaw it at room temperature, wash it 3 times with distilled water to remove the salt ions on the surface of the algae, and then use absorbent paper to dry the surface water. Weigh 1g of kelp root into a small beaker, add 3ml of DMSO, seal the mouth of the beaker with two layers of plastic wrap, extract in the dark for 50min, pour the extract into a brown bottle, wash the residue with 3ml of distilled water, and pour into a brown bottle. Determination of absorbance A 665 , A 631 , A 582 , A 480 , calculate the pigment concentration according to the following formula: [chl a]=A 665 / 72.8; [chl c]=(A 631 +A 582 -0.298A 665 ) / 61.8; [Fucoxanthin]=(A 480 -0.723 (A 631 +A 582 -0.298A 665 )-0.049A 665 ) / 130. The calculated yield is based on the amount of pigment proposed in each gram of kelp root, and the results are shown in Table 1.

[0038] deal with

Embodiment 2

[0040] Take out the fresh or frozen kelp root, thaw it at room temperature, wash it 3 times with distilled water to remove the salt ions on the surface of the algae, and then use absorbent paper to dry the surface water. Weigh 1g of kelp root into a small beaker, add 4ml of DMSO, seal the mouth of the beaker with two layers of plastic wrap, extract in the dark for 15 minutes, pour the extract into a brown bottle, and wash the residue with 4ml of distilled water Pour into a brown bottle. Absorbance measurement and calculation method of pigment concentration are the same as in Example 1, and the calculated yield is based on the amount of pigment proposed in each gram of kelp root. The results are shown in Table 2.

[0041] deal with

Embodiment 3

[0043] Take out the fresh or frozen kelp root, thaw it at room temperature, wash it 5 times with distilled water to remove the salt ions on the surface of the algae, and then use absorbent paper to dry the surface water. Weigh 1g of kelp root into a small beaker, add 7ml of DMSO, seal the mouth of the beaker with two layers of plastic wrap, extract in the dark for 30 minutes, pour the extract into a brown bottle, and wash the residue with 7ml of distilled water Pour into a brown bottle. The absorbance measurement and pigment concentration calculation method are the same as in Example 1, and the calculated yield is based on the amount of pigment proposed in each gram of kelp root. The results are shown in Table 3.

[0044] deal with

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Abstract

The present invention relates to the extraction and separation of fucoxanthin, and is especially the process of separating fucoxanthin from algae. The process of separating fucoxanthin from algae includes the following steps: cleaning fresh algae or defrozen algae to eliminate surface salt ion, eliminating surface water, leaching algae with dimethyl sulfoxide in 2-6 ml for each gram of algae in dark for 15-60 min, and final extracting coloring matter from dimethyl sulfoxide leached liquid with the mixture solution of ethyl acetate and ammonium sulfate. The present invention has the advantages of simple operation, high separation speed and high yield, and is suitable for industrial production.

Description

technical field [0001] The invention relates to the extraction and separation of fucoxanthin, in particular to a method for separating fucoxanthin from seaweed. Background technique [0002] Fucoxanthin (fucoxanthin, abbreviated as fucox herein), also known as fucoxanthin, is yellow-brown and is one of the important members of the carotenoid family, which has a very strong antioxidant effect. Molecular formula is C 40 h 60 o 6 ; The structural formula is: [0003] [0004] Fucoxanthin mainly exists in brown algae and diatoms, and as a peripheral light-harvesting pigment, the content in algal bodies is very large. As early as the 1960s, it was found that feeding poultry with seaweed food can deepen the color of egg yolk, and this effect is caused by the poultry absorbing the fucoxanthin in the algae. In recent years, Japanese scholars have found that fucoxanthin has strong anti-tumor activity in anti-tumor experimental research. Murakami's 2002 study showed that fuco...

Claims

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Application Information

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IPC IPC(8): C07D303/12
Inventor 王广策汪文俊王发左曾呈奎
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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