Compound active biological factor beauty liquid
A technology of biological factors and beauty serums, applied in the direction of pharmaceutical formulations, etc., can solve the problems of accelerating wound healing, cosmetics and other problems, and achieve the effect of reducing and removing scars, improving the repair effect, and accelerating the renewal.
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Embodiment 1
[0020] Compound active biological factor on skin cell doubling level (PDL) test:
[0021] Using DMEM / F12 medium, 37°C, 5% CO 2 In the incubator, EGF (1×10 -4 WT%), aFGF (1×10 -4 WT%), EGF+aFGF (each 1×10 -4 WT%), with the culture fluid as a control contrast, the doubling situation of the skin cells is measured, and the skin cell doubling level of the control contrast is 1, and the results of the survey are shown in figure 1 :
Embodiment 2
[0023] Making 10,000 sets of beauty lotion (freeze-dried type) of the present invention comprises the following steps:
[0024] (1) Get EGF300mg with a purity greater than 95%, add it to 30000ml of pure sterile water, use a 0.2um filter membrane to filter it aseptically, and pack (5ml vials) on a fully automatic, aseptically purified automatic filling line. 3ml vials, freeze-dried, stoppered and capped under GMP environment.
[0025] (2) Take 300 mg of aFGF with a purity greater than 95%, add it to 30000 ml of pure sterile water, filter it aseptically with a 0.2um filter membrane, and pack it on a fully automatic, aseptically purified automatic filling line (5ml vials), 3ml per bottle, freeze-dried, stoppered and capped under GMP environment.
[0026] (3) Dissolving solution preparation: prepare 20 liters of dissolving solution containing 3 (wt)% glycerin, 0.1 (wt)% hyaluronic acid, 0.01 (wt)% L-vitamin C sterile aqueous solution, after aseptic treatment, pack into 10,000 bo...
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