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Extracting process of actinidia proteinase

A technology of kiwifruit protease and extraction method, which is applied to the preparation methods of peptides, chemical instruments and methods, hydrolytic enzymes, etc., can solve the problems of no product sales, no public reports, etc., to fill market gaps and low equipment costs. , the effect of quick profit

Inactive Publication Date: 2005-01-05
SHAANXI INST OF ZOOLOGY NORTHWEST INSTOF ENDANGERED ZOOLOGICAL SPECIES
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Problems solved by technology

[0009] After retrieval, the extraction method that can be used for industrial production of kiwi fruit protease has not been seen in public reports, and there is no product sales

Method used

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  • Extracting process of actinidia proteinase

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0041] Example 1 Take 1 liter of kiwi fruit juice, add 1 liter of the prepared 25% sodium chloride aqueous solution, stir to mix well; adjust the pH to 4.0 with sodium hydroxide solution, and let it stand for 12 hours at 4°C (usually the same day). Juice in the afternoon, then let sit in the fridge overnight). After salting-out precipitation, the juice mixture is clearly separated into upper and lower layers, and the lower layer is the green enzyme paste sediment. The supernatant liquid of the upper layer was sucked out with a siphon; about 800 ml of the sediment in the lower layer was collected, transferred to a Buchner funnel and filtered under reduced pressure to obtain 53.6 g of wet crude enzyme product.

[0042] After 23.6 g of the wet enzyme is naturally spread out, vacuum drying is performed, and the drying temperature is not higher than 40° C., and finally the water content of the crude enzyme is below 5%. The dried crude enzyme was ground with a mortar and passed thr...

example 2

[0045] Example 2 Take 6 liters of kiwi fruit juice, add 4.3 liters of 28% saturated brine, and stir to fully mix the brine and juice. Adjust the pH to 4.3 with sodium hydroxide solution, transfer the mixture to a cold storage at 0°C, and let it settle for 14 hours until the supernatant is clear and transparent; remove the supernatant, and put the lower green enzyme paste into a 220-mesh filter. In the cloth bag, the brine is squeezed out to complete the solid-liquid separation.

[0046] Mix the above wet enzyme with 6 liters of purified water, adjust the pH value to 7.0, stir until completely dissolved, then adjust the pH to 4.6, and let stand for more than 3 hours in a 0°C cold storage; transfer to a centrifuge at 4000r / min speed Perform centrifugation. The precipitates were collected, 5 liters of 80% ethanol solution was added, and after thorough stirring, normal pressure filtration was performed, and then vacuum freeze-dried. Then, it is ground with a ball mill and passed...

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Abstract

The invention is a method of extracting proteinase of kiwi fruit, adopting the salt to make salting-out precipitation process on kiwi fruit juice, and using isoelectric precipitation process, by solid-liquid separation, obtaining a wet proteinase of kiwi fruit, drying and crushing to obtain the dry powder of the proteinase of kiwi fruit. It has advantages of simple process, low equipment cost and fast benefits. It can make industrialized production. It overcomes the defects in ammonium sulphate process and be applied to foods and medicines. And the whole process meets the requirements for green and clean production. The produced proteinase meets hygienic quality standard of foods additive by inspection. By the invention, it can deeply process the kiwi fruit, and increasing the add-on value of kiwi fruit and able to produce the product proteinase of kiwi fruit.

Description

technical field [0001] The invention belongs to the improvement of the method for extracting kiwifruit protease from kiwifruit fruit. Background technique [0002] In addition to being rich in vitamin C, polysaccharides, mineral elements and other nutrients, the ripe fruit of kiwifruit also contains kiwifruit protease (Actinidin) (Zhu Chen et al. Analysis of the nutritional components of kiwifruit fruits "Guangdong Pharmacy" 1996, 6(3)5- 6). [0003] Among the plants that have been found to have high content of protease (there are pineapple, papaya, fig, sisal, kiwi fruit), kiwifruit is one of them (Huang Huihua, etc., Research Overview of Plant Protease Resources. "Guangdong Food Industry Science and Technology" 1993, (2) 1-5). Kiwifruit protease has a wide range of uses in food, medicine, textile printing and dyeing, leather manufacturing, freckle beauty, biochemical reagents and other fields. In food, it can be used to clarify beer and prevent cold turbidity of beer (L...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/36C07K14/415C12N9/50
Inventor 左玉萍赵淑琳刘晓农
Owner SHAANXI INST OF ZOOLOGY NORTHWEST INSTOF ENDANGERED ZOOLOGICAL SPECIES
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