Production process of nostoc sphaeroides

A technology for algae and miscellaneous algae, which is applied in the field of preparation of algae seeds, can solve the problems of complex operation, small number of algal filaments, difficulty in adapting to large-scale cultivation of algae seeds, etc., and achieves a large number and a simple operation process. Effect

Inactive Publication Date: 2004-11-10
INST OF AQUATIC LIFE ACAD SINICA
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Problems solved by technology

This method is complicated to operate, requires antibiotics to be aseptically purified, and the algae preparation cycle is long, ...

Method used

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Embodiment Construction

[0059] A kind of preparation method of Gexian rice algae is carried out in the following steps:

[0060] 1. Collect seed algae: There are two ways to obtain seed algae, (1) wild Gexianmi population, (2) artificially cultivated Gexianmi population; for wild Gexianmi, if the collection time is within For more than 2 days, it can be dried first and stored for a long time. Before seed production, it can be fully soaked in culture medium to make it rehydrated into a spherical shape; It is in the logarithmic phase of the growth of Gexianmi group, and the algae with good surface gloss, dark color, fast growth speed and good group toughness are selected.

[0061] 2. Wash the spherical group of Ge Xianmi with water, soak in 70%-95% ethanol solution for 10-40 seconds, remove algae, and then wash 1-3 times in sterile water or culture solution.

[0062] 3. Put the spherical population of Gexianmi into a homogenizer, add culture medium 0.1-1.0 times the weight of the spherical population ...

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Abstract

The invention discloses a nostoc strain preparing method, selecting culture collection, washing and sterilizing, evening pulp, inoculating and cultivating, and obtaining a large number of nostoc strains. Compared with existing techniques, it has the advantages: simple operation, making aseptic purification without antibiotic, short cycle of preparing strains, high efficiency, therefore it can form a large number of strains, and meet the demand on strain preparation.

Description

Technical field: [0001] The invention relates to microalgae, and more specifically to a preparation method of the species of S. kudzui. Background technique: [0002] Nostoc belongs to Cyanophyta Cyanophyta Cyanophyceae Cyanophyceae segmental algae Hormogonales Nostocaceae, a class of algae with wide distribution, complex ecological environment, and diverse morphological structures. Its plants are gelatinous or leathery, and mature plants have Spherical, leafy, filamentous, vesicular and other shapes, hollow or solid, floating or growing. The original plant consists of many fine filaments with a diameter of a few microns assembled into a group, and the shape of the group varies depending on the "species", such as Nostoc flageliforme Kütz., Nostoc commune, Nostoc sphaeroides In the fresh state, they are hair-like, flat ear-shaped or small ball-shaped respectively, and they shrink to varying degrees after drying, all of which have rich nutritional value. , "Wuzhou Fuzhi", "N...

Claims

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Application Information

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IPC IPC(8): C12N1/12
Inventor 邓中洋胡征宇胡强
Owner INST OF AQUATIC LIFE ACAD SINICA
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