Method of detecting and quantifying human P450 molecular species and probe and kit for this method

Abstract

The present invention provides a kit for detecting and quantifying human cytochrome P450 molecular species, comprising one or more oligonucleotide probes each hybridizable with a specific region of the gene encoding a human cytochrome P450 species, for example, the 616 to 641 region of the CYP1A1 gene, and one or more specific primer pairs; and a method of detecting and quantifying one or more human cytochrome P450 molecular species using the kit.

Description

technical field [0001] The present invention relates to a method for detecting and quantifying human P450 molecular species, as well as probes and kits for the method. Background of the invention [0002] Cytochrome P450 is one of the enzymes involved in the metabolism of chemical substances such as active ingredient compounds in medicines. Numerous molecular species of cytochrome P450 are known to exist, and to date, about 30 molecular species have been identified in humans. Although these molecular species differ from each other in enzymatic activity, each of them has been reported to be involved in active ingredient compounds in medicines (such as tricyclic antidepressants, antiepileptics, diazepines, β-blockers, barbie active ingredient compounds in sleep-inducing hypnotics and other drugs, dimethylnitrosamine, etc.), benzene and other organic solvents, low molecular weight carcinogens in the environment, etc. (e.g. Yoo, J.S.H., Chung, R. , C., Wade, D., & Yang, C.S. (...

AI Technical Summary

Problems solved by technology

Specifically, although the ease of hybridization between two nucleic acids of known nucleotide sequence can be estimated to some extent by calculating the melting point (Tm), combinations of primers and probes There is no need to bring good results in